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Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma

  • Authors:
    • Makoto Arai
    • Fumio Imazeki
    • Yuzo Sakai
    • Rintaro Mikata
    • Motohisa Tada
    • Naohiko Seki
    • Hideaki Shimada
    • Takenori Ochiai
    • Osamu Yokosuka

  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/or_00000022
  • Pages: 405-412
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Abstract

To determine the clinical significance of gene promoter methylation in esophageal squamous cell carcinoma (ESCC), we examined the promoter methylation status of genes showing elevated expression, as determined by DNA microarray-based transcriptomic analysis, in three ESCC cell lines (TE-1, TE-2, TE-10) after 5-aza-2'-deoxycytidine (DAC) treatment. We observed a high degree of DNA methylation within the promoter regions of three genes, namely cathepsin L2 (CTSL2), normal mucosa of esophagus specific 1 (NMES1), and fatty acid binding protein 5 (FABP5). Overexpression of NMES1 in ESCC cell lines increased cell motility. Down-regulation of NMES1 might play an important role in the cell motility of ESCC or be a potent marker of malignancy.

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August 2008
Volume 20 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Journal Metrics
  • Impact Factor: 3.8
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Arai M, Imazeki F, Sakai Y, Mikata R, Tada M, Seki N, Shimada H, Ochiai T and Yokosuka O: Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma. Oncol Rep 20: 405-412, 2008.
APA
Arai, M., Imazeki, F., Sakai, Y., Mikata, R., Tada, M., Seki, N. ... Yokosuka, O. (2008). Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma. Oncology Reports, 20, 405-412. https://doi.org/10.3892/or_00000022
MLA
Arai, M., Imazeki, F., Sakai, Y., Mikata, R., Tada, M., Seki, N., Shimada, H., Ochiai, T., Yokosuka, O."Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma". Oncology Reports 20.2 (2008): 405-412.
Chicago
Arai, M., Imazeki, F., Sakai, Y., Mikata, R., Tada, M., Seki, N., Shimada, H., Ochiai, T., Yokosuka, O."Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma". Oncology Reports 20, no. 2 (2008): 405-412. https://doi.org/10.3892/or_00000022