Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells

  • Authors:
    • Zhongsheng Xia
    • Zhaohua Zhu
    • Liyong Zhang
    • Crista Royal
    • Zhongmin Liu
    • Qikui Chen
    • Bao-Ling Adam
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/or_00000163
  • Pages: 1433-1439
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

No control cell line was available for previous RNA interference studies on reversal of multidrug resistance (MDR) in colon cancer cells. Here, human COLO 320DM, with HT-29 as the control, colon cancer cell lines were used to investigate the reversal of MDR1/P-gp-dependent MDR by siRNA (#4123 and #4029 MDR1 siRNAs) targeting to MDR1 mRNA. Both siRNAs inhibited expression of MDR1 and P-gp in COLO 320DM. The minimum inhibition concentrations were 5 nmol/l of #4123 and 25 nmol/l of #4029. #4123 MDR1 siRNA took effect in 4, 5 and 6 days at doses of 5, 25 and 100 nmol/l, respectively. Increased cytotoxicity of the antitumor drugs adriamycin and vincristine with increased intracellular adriamycin accumulation accompanied inhibition of MDR1 mRNA and P-gp expression. No such effects were found in the HT-29 control. MDR1 siRNAs specifically reversed the MDR of colon cancer cells demonstrating a possible new approach for treating MDR1/P-gp-dependent multidrug resistance.

Related Articles

Journal Cover

December 2008
Volume 20 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xia Z, Zhu Z, Zhang L, Royal C, Liu Z, Chen Q and Adam B: Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells. Oncol Rep 20: 1433-1439, 2008.
APA
Xia, Z., Zhu, Z., Zhang, L., Royal, C., Liu, Z., Chen, Q., & Adam, B. (2008). Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells. Oncology Reports, 20, 1433-1439. https://doi.org/10.3892/or_00000163
MLA
Xia, Z., Zhu, Z., Zhang, L., Royal, C., Liu, Z., Chen, Q., Adam, B."Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells". Oncology Reports 20.6 (2008): 1433-1439.
Chicago
Xia, Z., Zhu, Z., Zhang, L., Royal, C., Liu, Z., Chen, Q., Adam, B."Specific reversal of MDR1/P-gp-dependent multidrug resistance by RNA interference in colon cancer cells". Oncology Reports 20, no. 6 (2008): 1433-1439. https://doi.org/10.3892/or_00000163