Overexpression of orotate phosphoribosyl transferase in hormone-refractory prostate cancer

  • Authors:
    • Tomoaki Tanaka
    • Hidenori Kawashima
    • Kentaro Matsumura
    • Tomoko Yamashita-Hosono
    • Rikio Yoshimura
    • Katsuyuki Kuratsukuri
    • Koji Harimoto
    • Tatsuya Nakatani
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/or_00000186
  • Pages: 33-37
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Abstract

Orotate phosphoribosyl transferase (OPRT) is the initial enzyme of 5-fluorouracil (5-FU) activation, in which 5-FU is converted to 5-fluorouridinemonophosphate. Dihydropyrimidine dehydrogenase (DPD) is a degrading enzyme that catabolizes 5-FU. In this study, we investigated the expression of these enzymes in normal prostate gland (NP), hormone-sensitive prostate cancer (HSPC) and hormone-refractory prostate cancer (HRPC). Forty-two prostatic tissue specimens were obtained from patients who had undergone prostate needle biopsies without any treatments or with PSA failure after initial androgen deprivation. The tissue samples derived from formalin-fixed, paraffin-embedded sections were made by laser-captured microdissection and from those RNA was extracted. The levels of OPRT and DPD mRNA expression were examined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The level of OPRT mRNA expression in the HSPC or the HRPC specimens was significantly higher than that in the NP specimens. Immunohistochemical staining for OPRT revealed strong expression of OPRT in prostate cancer cells. There was a significant correlation between OPRT mRNA expression levels and the tumor pathological grade. Furthermore, the OPRT/DPD expression ratio, a powerful predictive factor to evaluate 5-FU sensitivity, in the HRPC group was significantly higher than that in the low grade HSPC group. Thus, 5-FU may be an effective option for some HRPC patients.

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January 2009
Volume 21 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tanaka T, Kawashima H, Matsumura K, Yamashita-Hosono T, Yoshimura R, Kuratsukuri K, Harimoto K and Nakatani T: Overexpression of orotate phosphoribosyl transferase in hormone-refractory prostate cancer. Oncol Rep 21: 33-37, 2009.
APA
Tanaka, T., Kawashima, H., Matsumura, K., Yamashita-Hosono, T., Yoshimura, R., Kuratsukuri, K. ... Nakatani, T. (2009). Overexpression of orotate phosphoribosyl transferase in hormone-refractory prostate cancer. Oncology Reports, 21, 33-37. https://doi.org/10.3892/or_00000186
MLA
Tanaka, T., Kawashima, H., Matsumura, K., Yamashita-Hosono, T., Yoshimura, R., Kuratsukuri, K., Harimoto, K., Nakatani, T."Overexpression of orotate phosphoribosyl transferase in hormone-refractory prostate cancer". Oncology Reports 21.1 (2009): 33-37.
Chicago
Tanaka, T., Kawashima, H., Matsumura, K., Yamashita-Hosono, T., Yoshimura, R., Kuratsukuri, K., Harimoto, K., Nakatani, T."Overexpression of orotate phosphoribosyl transferase in hormone-refractory prostate cancer". Oncology Reports 21, no. 1 (2009): 33-37. https://doi.org/10.3892/or_00000186