Cell growth regulation through apoptosis by activin in human gastric cancer SNU-16 cell lines

  • Authors:
    • Young Il Kim
    • Byung-Ho Kim
    • Inkoo Khang
    • Byung-Nam Cho
    • Ha Kyu Lee
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/or_00000249
  • Pages: 491-497
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Abstract

Activin has a wide variety of biological functions, including the regulation of cell proliferation and inhibition of tumor cells. We have studied whether activin regulates apoptosis by investigating the effects of activin A on cell proliferation, cell cycle, apoptosis, apoptosis-related gene expression, and caspase activity in SNU-16 cells. Activin A significantly inhibited DNA synthesis and growth suppression in a time-dependent manner in SNU-16 cells. Apoptosis fraction was increased at cell cycle with an accompanying DNA fragmentation. Activin A resulted in a significant time-dependent decrease in Bcl-2 mRNA levels and increase in caspase-3 mRNA levels in SNU-16 cells. No significant difference was observed in Bax mRNA levels. Exposure of cells to activin A induced caspase-3, -8 and -9 activation in SNU-16 cells. Furthermore, co-treatment of activin with the pan-caspase inhibitor Z-VAD-FMK, caspase-3 inhibitor Z-DEVE-FMK, caspase-8 inhibitor Z-IETD-FMK, and caspase-9-inhibitor Z-LEHD-FMK blocked apoptosis of SNU-16 cells. Taken together, our results revealed that activin inhibits the growth of SNU-16 cells by inducing apoptosis through caspase activation.

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February 2009
Volume 21 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim YI, Kim B, Khang I, Cho B and Lee HK: Cell growth regulation through apoptosis by activin in human gastric cancer SNU-16 cell lines. Oncol Rep 21: 491-497, 2009.
APA
Kim, Y.I., Kim, B., Khang, I., Cho, B., & Lee, H.K. (2009). Cell growth regulation through apoptosis by activin in human gastric cancer SNU-16 cell lines. Oncology Reports, 21, 491-497. https://doi.org/10.3892/or_00000249
MLA
Kim, Y. I., Kim, B., Khang, I., Cho, B., Lee, H. K."Cell growth regulation through apoptosis by activin in human gastric cancer SNU-16 cell lines". Oncology Reports 21.2 (2009): 491-497.
Chicago
Kim, Y. I., Kim, B., Khang, I., Cho, B., Lee, H. K."Cell growth regulation through apoptosis by activin in human gastric cancer SNU-16 cell lines". Oncology Reports 21, no. 2 (2009): 491-497. https://doi.org/10.3892/or_00000249