PGF isoforms, PLGF-1 and PGF-2 and the PGF receptor, neuropilin, in human breast cancer: Prognostic significance

  • Authors:
    • Astrid Escudero-Esparza
    • Tracey A. Martin
    • Anthony Douglas-Jones
    • Robert E. Mansel
    • Wen G. Jiang
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/or_00000667
  • Pages: 537-544
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Abstract

Placenta growth factor (PLGF) is a member of the vascular endothelial growth factor (VEGF) family, a group of angiogenic growth factors. Recently, isoforms have been identified. This study examined PLGF-1, PGF-2 and its receptor neuropilin-1 levels in human breast cancer in relation to patient's clinical parameters and how changes in expression may be linked to prognosis of the disease. PLGF-1, PGF-2 and neuropilin-1 transcript expression and distribution were examined quantitatively using real-time quantitative polymerase chain reaction (Q-PCR) on a cohort of human breast cancer (n=114) and background breast tissue (n=30) with a 10-year follow-up. Protein expression was assessed by an immunohistochemical method. We demonstrate that PLGF-1 transcript levels were significantly elevated when comparing tumours from patients with poor outcome and patients who remained disease-free (P=0.03), indicating a potential prognostic value. Immunohistochemistry demonstrated a marked increased in PGF-2 expression in tumour section compared with normal tissues (P<0.05). PGF-2 transcripts, showed little change in expression between tumour and background. High levels of PLGF-1 and PGF-2 were seen in ERβ-negative breast tumour tissues. Neuropilin transcript was below detection in substantial portion of the samples and was more frequently detected in high grade tumours (P=0.008 vs. low grade) and in tumours from patients who died of breast cancer (P<0.001 vs. those who remained disease-free). Our study shows that PLGF isoforms PLGF-1 and PGF-2 and indeed their receptor neuopilin, have an aberrant pattern of expression and that high levels of the PLGF-1 and neuropilin are linked to a poor prognosis.

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February 2010
Volume 23 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Escudero-Esparza A, Martin TA, Douglas-Jones A, Mansel RE and Jiang WG: PGF isoforms, PLGF-1 and PGF-2 and the PGF receptor, neuropilin, in human breast cancer: Prognostic significance. Oncol Rep 23: 537-544, 2010.
APA
Escudero-Esparza, A., Martin, T.A., Douglas-Jones, A., Mansel, R.E., & Jiang, W.G. (2010). PGF isoforms, PLGF-1 and PGF-2 and the PGF receptor, neuropilin, in human breast cancer: Prognostic significance. Oncology Reports, 23, 537-544. https://doi.org/10.3892/or_00000667
MLA
Escudero-Esparza, A., Martin, T. A., Douglas-Jones, A., Mansel, R. E., Jiang, W. G."PGF isoforms, PLGF-1 and PGF-2 and the PGF receptor, neuropilin, in human breast cancer: Prognostic significance". Oncology Reports 23.2 (2010): 537-544.
Chicago
Escudero-Esparza, A., Martin, T. A., Douglas-Jones, A., Mansel, R. E., Jiang, W. G."PGF isoforms, PLGF-1 and PGF-2 and the PGF receptor, neuropilin, in human breast cancer: Prognostic significance". Oncology Reports 23, no. 2 (2010): 537-544. https://doi.org/10.3892/or_00000667