Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells

  • Authors:
    • Ning Zhang
    • Ren Li
    • Kai-Shan Tao
    • Da-Yong Cao
    • Zhen-Yu Ti
    • Rui Ding
    • Lei Cai
    • Fu-Qin Zhang
    • Ke-Feng Dou
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/or_00000704
  • Pages: 827-831
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Abstract

A small stem-like subpopulation was isolated from human hepatocellular carcinoma (HCC) MHCC97 cells, characterized by their high efflux ability of the Hoechst 33342 dye. These side population (SP) cells were able to generate a heterogeneous mixture of SP and main population (MP) cells, while the MP cells rarely generated SP cells. Cell cycle analysis also revealed that more SP cells were in the G0 phase. They express higher levels of BCRP1, AFP and CK19 than MP cells. SP cells showed significantly higher viability than MP cells following treatment with doxorubicin or methotrexate. Actin polymerization and migration assays indicate that SP cells have a higher migration capacity and in vivo tumorigenicity of these cells is also higher. Collectively, we conclude that the SP is an enriched source of stem-like cells and may be an effective target for therapy and a useful tool to investigate the HCC tumorigenic and metastatic process.

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March 2010
Volume 23 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang N, Li R, Tao K, Cao D, Ti Z, Ding R, Cai L, Zhang F and Dou K: Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells. Oncol Rep 23: 827-831, 2010.
APA
Zhang, N., Li, R., Tao, K., Cao, D., Ti, Z., Ding, R. ... Dou, K. (2010). Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells. Oncology Reports, 23, 827-831. https://doi.org/10.3892/or_00000704
MLA
Zhang, N., Li, R., Tao, K., Cao, D., Ti, Z., Ding, R., Cai, L., Zhang, F., Dou, K."Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells". Oncology Reports 23.3 (2010): 827-831.
Chicago
Zhang, N., Li, R., Tao, K., Cao, D., Ti, Z., Ding, R., Cai, L., Zhang, F., Dou, K."Characterization of a stem-like population in hepatocellular carcinoma MHCC97 cells". Oncology Reports 23, no. 3 (2010): 827-831. https://doi.org/10.3892/or_00000704