Synergistic gastric cancer inhibition by chemogenetherapy with recombinant human adenovirus p53 and epirubicin: an in vitro and in vivo study

  • Authors:
    • Yi-Shan Xie
    • Yan-Hua Zhang
    • Shao-Ping Liu
    • Shi-Quan Liu
    • Chun-Wei Peng
    • Long Wu
    • He-Shen Luo
    • Yan Li
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/or_00001025
  • Pages: 1613-1620
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Abstract

This study was desinged to investigate the in vitro and in vivo antitumor effect on SGC-7901 gastric cancer cells by chemogenetherapy. SGC-7901 cells were treated by chemogenetherapy with Gendicine, a recombinant human Ad-p53 injection (rAd-p53), and epirubicin hydrochloride (EPI), a cytotoxic chemotherapy agent. Compared with blank control, rAd-p53, EPI, and combined therapy achieved SGC-7901 growth inhibition by 32.26, 35.48, 43.44%, respectively on day 1 and 70.62, 78.82, 87.15%, respectively on day 2 (rAd-p53, EPI VS control, p<0.01; rAd-p53+EPI VS either alone, p<0.05). Flow cytometry study confirmed that rAd-p53 and/or EPI mainly inhibit the cell cycle at S phase. SGC-7901 cells were subcutaneously injected into the nude mice to form xenograft models, which were treated with rAd-p53 and EPI. Compared with the blank control, treatment with rAd-p53 at the dose of 10 µl of 1012 vp/ml and EPI at the dose of 1.25 mg/kg, 7 times in 3 weeks, resulted in 80 and 60% of tumor growth inhibition, respectively. No animal death was observed, although 2 nude mice in rAd-p53 group developed liver toxicity and 1 nude mouse in EPI group developed cardiac toxicity. rAd-p53 and EPI have synergistic tumor inhibition effect on gastric cancer cells.

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December 2010
Volume 24 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xie Y, Zhang Y, Liu S, Liu S, Peng C, Wu L, Luo H and Li Y: Synergistic gastric cancer inhibition by chemogenetherapy with recombinant human adenovirus p53 and epirubicin: an in vitro and in vivo study . Oncol Rep 24: 1613-1620, 2010.
APA
Xie, Y., Zhang, Y., Liu, S., Liu, S., Peng, C., Wu, L. ... Li, Y. (2010). Synergistic gastric cancer inhibition by chemogenetherapy with recombinant human adenovirus p53 and epirubicin: an in vitro and in vivo study . Oncology Reports, 24, 1613-1620. https://doi.org/10.3892/or_00001025
MLA
Xie, Y., Zhang, Y., Liu, S., Liu, S., Peng, C., Wu, L., Luo, H., Li, Y."Synergistic gastric cancer inhibition by chemogenetherapy with recombinant human adenovirus p53 and epirubicin: an in vitro and in vivo study ". Oncology Reports 24.6 (2010): 1613-1620.
Chicago
Xie, Y., Zhang, Y., Liu, S., Liu, S., Peng, C., Wu, L., Luo, H., Li, Y."Synergistic gastric cancer inhibition by chemogenetherapy with recombinant human adenovirus p53 and epirubicin: an in vitro and in vivo study ". Oncology Reports 24, no. 6 (2010): 1613-1620. https://doi.org/10.3892/or_00001025