Analysis of plasma free amino acid profiles in canine brain tumors

  • Authors:
    • Shinichi Utsugi
    • Kazuo Azuma
    • Tomohiro Osaki
    • Yusuke Murahata
    • Takeshi Tsuka
    • Norihiko Ito
    • Tomohiro Imagawa
    • Yoshiharu Okamoto
  • View Affiliations

  • Published online on: December 6, 2016     https://doi.org/10.3892/br.2016.825
  • Pages: 195-200
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Abstract

Canine brain tumors are best diagnosed using magnetic resonance imaging (MRI). However, opportunities of MRI examination are restricted due to its limited availability in veterinary facilities; thus, numerous canine brain tumors are diagnosed at an advanced stage. Therefore, development of a noninvasive diagnostic biomarker is required for the early detection of brain tumors. In the present study, plasma free amino acid (PFAA) profiles between dogs with and without brain tumors were compared. A total of 12 dogs with brain tumors, diagnosed based on clinical signs, and on the results of intracranial MRI and/or pathological examination were evaluated. In addition, eight dogs diagnosed with idiopathic epilepsy and 16 healthy dogs were also included. A liquid chromatography system with automated pre‑column derivatization functionality was used to measure the levels of 20 amino acids. As a result, the levels of three amino acids (alanine, proline and isoleucine) were increased significantly (1.6‑, 1.5‑ and 1.6‑fold, respectively) in the plasma of dogs with brain tumors as compared with the levels in control dogs (all P<0.05). Thus, the PFAA levels of dogs with brain tumors differed from those of healthy dogs. The present study demonstrated that analysis of PFAA levels of dogs with brain tumors may serve as a useful biomarker for the early detection of canine brain tumors.
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February-2017
Volume 6 Issue 2

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Utsugi S, Azuma K, Osaki T, Murahata Y, Tsuka T, Ito N, Imagawa T and Okamoto Y: Analysis of plasma free amino acid profiles in canine brain tumors. Biomed Rep 6: 195-200, 2017
APA
Utsugi, S., Azuma, K., Osaki, T., Murahata, Y., Tsuka, T., Ito, N. ... Okamoto, Y. (2017). Analysis of plasma free amino acid profiles in canine brain tumors. Biomedical Reports, 6, 195-200. https://doi.org/10.3892/br.2016.825
MLA
Utsugi, S., Azuma, K., Osaki, T., Murahata, Y., Tsuka, T., Ito, N., Imagawa, T., Okamoto, Y."Analysis of plasma free amino acid profiles in canine brain tumors". Biomedical Reports 6.2 (2017): 195-200.
Chicago
Utsugi, S., Azuma, K., Osaki, T., Murahata, Y., Tsuka, T., Ito, N., Imagawa, T., Okamoto, Y."Analysis of plasma free amino acid profiles in canine brain tumors". Biomedical Reports 6, no. 2 (2017): 195-200. https://doi.org/10.3892/br.2016.825