Effect of trehalose on cryopreservation of pure peripheral blood stem cells

Martinetti,Daniela; Colarossi,Cristina; Buccheri,Simona; Denti,Gabriella; Memeo,Lorenzo; Vicari,Luisa

doi:10.3892/br.2017.859

Effect of trehalose on cryopreservation of pure peripheral blood stem cells

Authors:
- Daniela Martinetti
- Cristina Colarossi
- Simona Buccheri
- Gabriella Denti
- Lorenzo Memeo
- Luisa Vicari
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Affiliations: Cell Biology Unit, IOM Ricerca Srl, Viagrande, I‑95029 Catania, Italy, Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, I‑95029 Catania, Italy
Published online on: February 14, 2017 https://doi.org/10.3892/br.2017.859
Pages: 314-318

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Abstract

Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post‑thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thawing. Thus, the use of cryoprotectants is essential. In particular, the efficacy of trehalose has been reported for clinical purposes in blood stem cells. The aim of the current study was to establish an efficient method for biological research based on the use of trehalose, to cryopreserve pure peripheral blood stem cells. The efficacy of trehalose was assessed in vitro and the cell viability was evaluated. The data indicate that trehalose improves cell survival after thawing compared with the standard freezing procedure. These findings could suggest the potential for future trehalose application for research purposes in cell cryopreservation.

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