Proteomic profile of the lens in a streptozotocin‑induced diabetic rat model using shotgun proteomics

Nagai,Noriaki; Yamamoto,Tetsushi; Mitamura,Kuniko; Taga,Atsushi

doi:10.3892/br.2017.988

Proteomic profile of the lens in a streptozotocin‑induced diabetic rat model using shotgun proteomics

Authors:
- Noriaki Nagai
- Tetsushi Yamamoto
- Kuniko Mitamura
- Atsushi Taga
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Affiliations: Department of Advanced Design for Pharmaceuticals, Faculty of Pharmacy, Kindai University, Higashi‑Osaka, Osaka 577‑8502, Japan, Pathological and Biomolecule Analyses Laboratory, Faculty of Pharmacy, Kindai University, Higashi‑Osaka, Osaka 577‑8502, Japan
Published online on: September 22, 2017 https://doi.org/10.3892/br.2017.988
Pages: 445-450

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Abstract

Streptozotocin (STZ)‑induced diabetic rats (STZ rats) were used to investigate diabetic cataracts. In the current study, a shotgun liquid chromatography (LC)/mass spectrometry (MS)‑based global proteomic analysis method was used to examine the mechanism of lens opacification as a result of hyperglycemia in STZ rats. The 6‑week old Wistar rats were injected with STZ for 2 days (100 mg/kg/day, i.p.) and housed for 3 weeks. The plasma glucose levels were identified to be significantly higher when compared with the normal rats and insulin was not detected in the STZ rats. Furthermore, opacification of the cortical epithelium was observed in the lenses of STZ rats. A total of 235 proteins were identified in the lenses of the STZ rats and 229 in the lenses of the normal rats. A label‑free semi‑quantitative method, based on spectral counting, identified 52 proteins that were differentially expressed in the lenses of STZ rats compared with normal rats. In particular, superoxide dismutase, which is a critical antioxidant enzyme that detoxifies superoxide through redox cycling, was downregulated when analyzed by the semi‑quantitative method. In addition, phosphorylated‑p38, which is important in the signaling pathway involved in the oxidative stress response, was significantly increased in the lenses of STZ rats when compared with normal rats (P<0.05). Thus, the changes in protein expression were evaluated in the lenses of STZ rats using a shotgun LC/MS‑based global proteomic analysis approach, and a decrease in antioxidant enzymes and an increase in oxidative stress were identified in the lenses of STZ rats. Further studies are required to examine the role of these proteins in the onset or progression of diabetic cataracts.

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