Open Access

Toll‑like receptor 2 agonist exacerbates renal injury in diabetic mice

  • Authors:
    • Fanglin Li
    • Ningyu Zhang
    • Zhiming Li
    • Lihua Deng
    • Jianjie Zhang
    • Yunfeng Zhou
  • View Affiliations

  • Published online on: January 5, 2017     https://doi.org/10.3892/etm.2017.4031
  • Pages: 495-502
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inflammation is implicated in the pathogenesis of diabetic nephropathy (DN). Toll‑like receptor 2 (TLR2) is a ligand‑activated membrane‑bound receptor, which induces an inflammatory response, thus serving a crucial role in the pathogenesis of DN. The present study aimed to determine whether a TLR2 agonist, Pam3CysSK4, modulates the development of DN. A mouse model of DN was induced using streptozotocin (STZ) and, following the confirmation of hyperglycemia, mice were treated with or without Pam3CysSK4. Pathological and functional markers, including the activation of nuclear factor (NF)‑κB, expression of TLR2, inflammatory infiltration, myeloid differentiation primary response gene 88 and monocyte chemoattractant protein‑1 were assessed. STZ‑treated mice exhibited elevated blood glucose levels and increased serum creatinine levels, which increased further following Pam3CysSK4 treatment. In addition, Pam3CysSK4 treatment was observed to increase podocyte foot process formation. Furthermore, STZ‑induced renal glomerular sclerosis was significantly exacerbated in Pam3CysSK4‑treated mice. Pam3CysSK4‑treated mice also exhibited increased levels of collagen IV following renal immunostaining, associated with increased macrophage infiltration. Renal expression of TLR2 was markedly elevated in STZ‑induced mice; this was further increased in Pam3CysSK4‑treated mice, accompanied by upregulation of proinflammatory genes and activation of NF‑κB. This indicates that enhanced renal expression of TLR2 is associated with inflammatory infiltration in DN and demonstrates that renal injury was exacerbated by the TLR2 agonist in diabetic mice.

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February-2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li F, Zhang N, Li Z, Deng L, Zhang J and Zhou Y: Toll‑like receptor 2 agonist exacerbates renal injury in diabetic mice. Exp Ther Med 13: 495-502, 2017
APA
Li, F., Zhang, N., Li, Z., Deng, L., Zhang, J., & Zhou, Y. (2017). Toll‑like receptor 2 agonist exacerbates renal injury in diabetic mice. Experimental and Therapeutic Medicine, 13, 495-502. https://doi.org/10.3892/etm.2017.4031
MLA
Li, F., Zhang, N., Li, Z., Deng, L., Zhang, J., Zhou, Y."Toll‑like receptor 2 agonist exacerbates renal injury in diabetic mice". Experimental and Therapeutic Medicine 13.2 (2017): 495-502.
Chicago
Li, F., Zhang, N., Li, Z., Deng, L., Zhang, J., Zhou, Y."Toll‑like receptor 2 agonist exacerbates renal injury in diabetic mice". Experimental and Therapeutic Medicine 13, no. 2 (2017): 495-502. https://doi.org/10.3892/etm.2017.4031