Open Access

The mechanism of blood concentrations of the Shenqi pill repairing injured epithelial cells of renal tubular in vitro

  • Authors:
    • Fei Huang
    • Wenfeng Ma
  • View Affiliations

  • Published online on: May 11, 2017     https://doi.org/10.3892/etm.2017.4450
  • Pages:246-250
0

Abstract

In the present study, we investigated the best blood concentration of the mechanism of Shenqi pill repairing the injured epithelial cells of renal tubular in vitro. First, the injured hypoxia/reoxygenation model of rat proximal renal tubular epithelial cell strain (NRK-52E) was established. The animals were divided randomly into control, model, low concentration (5 µg/ml), moderate concentration (10 µg/ml) and high concentration (20 µg/ml) groups. The apoptotic rate was measured with flow cytometry and Jag2/Notch2/hes1 mRNA, and the protein expression was measured for 1, 3 and 7 days. It was found that in comparison to the control group, the growth of each group was prolonged with time, the levels of apoptosis, and the Jag2/Notch2/hes1 mRNA and protein expression decreased. Furthermore, the levels of the apoptotic rate, Jag2/Notch2/hes1 mRNA and protein expression of the moderate concentration and high concentration groups were significantly lower than those of the model and low‑dose groups at each time‑point (P<0.05). In conclusion, the Shenqi Pill alleviates the damage of renal tubular epithelial cells by inhibiting the Jag2/Notch2/hes1 signaling pathway; suitable concentration such as 10-20 µg/ml can exert protective effect.

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July 2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Huang, F., & Huang, F. (2017). The mechanism of blood concentrations of the Shenqi pill repairing injured epithelial cells of renal tubular in vitro. Experimental and Therapeutic Medicine, 14, 246-250. https://doi.org/10.3892/etm.2017.4450
MLA
Huang, F., Ma, W."The mechanism of blood concentrations of the Shenqi pill repairing injured epithelial cells of renal tubular in vitro". Experimental and Therapeutic Medicine 14.1 (2017): 246-250.
Chicago
Huang, F., Ma, W."The mechanism of blood concentrations of the Shenqi pill repairing injured epithelial cells of renal tubular in vitro". Experimental and Therapeutic Medicine 14, no. 1 (2017): 246-250. https://doi.org/10.3892/etm.2017.4450