Comparison of the gas‑liquid dual support fixation and Heitzman fixation techniques for preparing lung specimens

Yu,Dongsheng; Qu,Weili; Xia,Haipeng; Li,Xiaofeng; Luan,Zhenfeng; Yan,Renjie; Lu,Xiaodong; Zhao,Peng

doi:10.3892/etm.2017.4563

Comparison of the gas‑liquid dual support fixation and Heitzman fixation techniques for preparing lung specimens

Authors:
- Dongsheng Yu
- Weili Qu
- Haipeng Xia
- Xiaofeng Li
- Zhenfeng Luan
- Renjie Yan
- Xiaodong Lu
- Peng Zhao
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Affiliations: Department of Intervention, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China, Department of Cardiac Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Orthopedics, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China, Department of Intervention, Beijing Tumor Hospital, Beijing 100036, P.R. China, Department of Radiology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
Published online on: June 8, 2017 https://doi.org/10.3892/etm.2017.4563
Pages: 825-830

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Abstract

The aim of the present study was to compare the gas-liquid dual support fixation and Heitzman fixation techniques for the preparation of lung specimens. A total of 40 fresh lung samples were surgically collected from 40 male patients with lung cancer by biopsy. Patients were recruited from the Affiliated Hospital of Qingdao University Medical College (Qingdao, China) between July 2007 and June 2014. Samples were prepared using either the gas‑liquid dual support fixation method (group A; n=26) or the Heitzman fixation method (group B; n=14). High‑resolution computed tomography (HRCT) scanning was performed prior to surgery and corresponding postoperative HRCT scanning was conducted for the lung specimens; the gross transverse specimen section, cord photography images and histological sections were evaluated. Morphological observations of lung specimens indicated that there were 22 cases in group A with grade I (84.6%) and 4 cases with grade II (15.4%), whereas, in group B, there were 5 cases with grade II (35.7%) and 9 cases with grade III (64.3%). Statistical analysis demonstrated that the grades of specimens between the two groups were significantly different (P<0.01). Results from imaging and histological studies found that the quality of lung specimens was superior in group A, compared with group B. In conclusion, the present study demonstrated that, compared with the Heitzman fixation method, gas‑liquid dual support fixation may be a superior technique for the preparation of lung specimens. This finding may facilitate the improvement of lung HRCT and pathological studies.

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