Recombinant PEP‑1‑SOD1 improves functional recovery after neural stem cell transplantation in rats with traumatic brain injury

Jia,Jinming; Chen,Feifei; Wu,Yunfei

doi:10.3892/etm.2018.5781

Recombinant PEP‑1‑SOD1 improves functional recovery after neural stem cell transplantation in rats with traumatic brain injury

Authors:
- Jinming Jia
- Feifei Chen
- Yunfei Wu
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Affiliations: Department of Critical Care Medicine, The Putian Hanjiang Hospital, Putian, Fujian 351100, P.R. China, Department of Emergency, The Third People's Hospital of Changzhou, Changzhou, Jiangsu 213001, P.R. China, Department of Pathology, The Third People's Hospital of Changzhou, Changzhou, Jiangsu 213001, P.R. China
Published online on: January 22, 2018 https://doi.org/10.3892/etm.2018.5781
Pages: 2929-2935
Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The transplantation of neural stem cells (NSCs) has been demonstrated as a potential treatment strategy for traumatic brain injury (TBI). Cu,Zn‑superoxide dismutase (SOD1) is an important antioxidant enzyme that detoxifies intracellular reactive oxygen species, thereby protecting cells from oxidative damage. PEP‑1, a peptide carrier, is able to deliver full‑length native peptides or proteins into cells. Therefore, the current study investigated the effect of the transplantation of NSCs in combination with PEP‑1‑SOD1 for the treatment of experimental TBI in rats. Initially, the effect of PEP‑1‑SOD1 on the proliferation of NSCs was evaluated by MTT assay. PEP‑1‑SOD1 (0.5, 2.5 and 4.5 µM) significantly increased the proliferation rates of NSCs at 24, 48 and 72 h in a dose‑dependent manner. PEP‑1‑SOD1 also promoted the differentiation of NSCs in vitro. The in vivo experiment showed that PEP‑1‑SOD1 in combination with NSC transplantation significantly improved the functional recovery of rats following TBI compared with NSC transplantation alone. A significant increase in brain aquaporin‑4 (AQP4) mRNA and protein expression levels was observed 4 days post‑TBI in PEP‑1‑SOD1, NSCs and PEP‑1‑SOD1 + NSCs groups compared with the saline group. The PEP‑1‑SOD1 + NSCs group showed a further increase of AQP4 mRNA and protein expression levels compared with the NSCs and PEP‑1‑SOD1 groups. In conclusion, the current data suggests that PEP‑1‑SOD1 may promote the proliferation and differentiation of NSCs, and thereby improve the functional recovery of TBI model rats following NSCs transplantation through upregulating the expression of AQP4.

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