Open Access

Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium

  • Authors:
    • Hongbo Wang
    • Xiaolong Wang
    • Yan Li
    • Hao Yu
    • Changliang Wang
    • Chunmei Feng
    • Guohui Xu
    • Jiajun Chen
    • Jiabin You
    • Pengfei Wang
    • Xu Wu
    • Rui Zhao
    • Guohua Zhang
  • View Affiliations

  • Published online on: February 28, 2018     https://doi.org/10.3892/etm.2018.5902
  • Pages: 3791-3800
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has been identified that chronic ethanol exposure damages the nervous system, particularly neurons. There is scientific evidence suggesting that neuronal loss caused by chronic ethanol exposure has an association with neuron apoptosis and intracellular calcium oscillation is one of the primary inducers of apoptosis. Therefore, the present study aimed to investigate the inductive effects of intracellular calcium oscillation on apoptosis in SK‑N‑SH human neuroblastoma cells and the protective effects of the N‑methyl‑D‑aspartic acid receptor (NMDAR) antagonist, memantine, on SK‑N‑SH cell apoptosis caused by chronic ethanol exposure. SK‑N‑SH cells were treated with 100 mM ethanol and memantine (4 µM) for 2 days. Protein expression of NR1 was downregulated by RNA interference (RNAi). Apoptosis was detected by Annexin V/propidium iodide (PI) double‑staining and flow cytometry and cell viability was detected using an MTS kit. Fluorescence dual wavelength spectrophotometry was used to determine the intracellular calcium concentration and the levels of NR1 and caspase‑3 were detected using western blotting. NR1 mRNA levels were also detected using qPCR. It was found that chronic ethanol exposure reduced neuronal cell viability and caused apoptosis of SK‑N‑SH cells, and the extent of damage in SK‑N‑SH cells was associated with ethanol exposure concentration and time. In addition, chronic ethanol exposure increased the concentration of intracellular calcium in SK‑N‑SH cells by inducing the expression of NMDAR, resulting in apoptosis, and memantine treatment reduced ethanol‑induced cell apoptosis. The results of the present study indicate that the application of memantine may provide a novel strategy for the treatment of alcoholic dementia.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang H, Wang X, Li Y, Yu H, Wang C, Feng C, Xu G, Chen J, You J, Wang P, Wang P, et al: Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium. Exp Ther Med 15: 3791-3800, 2018.
APA
Wang, H., Wang, X., Li, Y., Yu, H., Wang, C., Feng, C. ... Zhang, G. (2018). Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium. Experimental and Therapeutic Medicine, 15, 3791-3800. https://doi.org/10.3892/etm.2018.5902
MLA
Wang, H., Wang, X., Li, Y., Yu, H., Wang, C., Feng, C., Xu, G., Chen, J., You, J., Wang, P., Wu, X., Zhao, R., Zhang, G."Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium". Experimental and Therapeutic Medicine 15.4 (2018): 3791-3800.
Chicago
Wang, H., Wang, X., Li, Y., Yu, H., Wang, C., Feng, C., Xu, G., Chen, J., You, J., Wang, P., Wu, X., Zhao, R., Zhang, G."Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3791-3800. https://doi.org/10.3892/etm.2018.5902