Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats

Zheng,Xuefeng; Zhang,Yuanyuan; Guo,Shiming; Zhang,Wenming; Wang,Jinyun; Lin,Yanping

doi:10.3892/etm.2018.6356

Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats

Authors:
- Xuefeng Zheng
- Yuanyuan Zhang
- Shiming Guo
- Wenming Zhang
- Jinyun Wang
- Yanping Lin
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Affiliations: Acupuncture College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Orthopedics Department, Zhangzhou Hospital of Traditional Chinese Medicine, Zhangzhou, Fujian 363000, P.R. China, Orthopedics Department, Luoyang Osteopathy Hospital, Luoyang, Henan 471002, P.R. China
Published online on: June 27, 2018 https://doi.org/10.3892/etm.2018.6356
Pages: 1807-1813
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the dynamic expression of matrix metalloproteinase (MMP)‑2, MMP‑9 and MMP‑13 in an ovariectomy (OVX)‑induced osteoporosis rat model. A total of 80 Sprague‑Dawley female rats (age, 3 months) were randomly divided into the OVX and sham groups, with 40 rats in each group. Rats in the sham group received sham surgery, while the remaining rats were ovariectomized. After 12, 16, 20 and 24 weeks, 10 rats from each group were randomly sacrificed, respectively. It was observed that the bone mineral density (BMD) and the trabecular bone area in the OVX group were significantly lower as compared with those in the sham group (P<0.01). The expression levels of MMP‑2 and MMP‑9 were negatively correlated with the BMD, while MMP‑13 was positively correlated with the BMD. The expression levels of MMP‑2 and MMP‑9 increased more abruptly and were significant higher in the OVX group in comparison with those in the sham group between 12 and 24 weeks after surgery (P<0.01). More specifically, the MMP‑9 mRNA expression level in the OVX group increased abruptly between 12 and 24 weeks after surgery. By contrast, in the sham group, the MMP‑9 mRNA level was undetectable between 12 and 16 weeks, and increased steadily between 16 and 24 weeks. Furthermore, the mRNA and protein expression levels of MMP‑13 initially increased and then decreased in the OVX group (P<0.01 vs. the sham group), whereas they continuously increased in the sham group between 12 and 24 weeks after surgery. In conclusion, MMP‑2, MMP‑9 and MMP‑13 regulated the development of osteoporosis, and MMP‑9 may be used as an important marker in the early diagnosis of osteoporosis.

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