Growth inhibitory potential of effective microorganism fermentation extract (EM-X) on cancer cells

  • Authors:
    • Chung Hin Chui
    • Gregory Yin Ming Cheng
    • Bin Ke
    • Fung Yi Lau
    • Raymond Siu Ming Wong
    • Stanton Hon Lung Kok
    • Sarwat Fatima
    • Filly Cheung
    • Chor Hing Cheng
    • Albert Sun Chi Chan
    • Johnny Cheuk On Tang
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijmm.14.5.925
  • Pages: 925-929
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Abstract

The effective microorganism (EM-X) fermentation extract is derived from rice bran and seaweed extract. It has been shown to possess anti-oxidation activity both in vitro and in vivo. To our knowledge, the possible in vitro anti-cancer potential of EM-X has not been demonstrated. Here we showed that the double concentrate of EM-X (EM-X2) at concentrations of 20-30% by volume, had growth inhibitory activity on MDA-MB231 breast cancer cell line and K-562 chronic myelogenous leukaemia cell lines by [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2-H-tetrazolium, inner salt] (MTS) assay. No characteristic features of apoptosis could be observed morphologically. Colony formation assay illustrated that both MDA-MB231 breast cancer and K-562 CML cells lost part of their regeneration potential after treatment with EM-X2 at 30% concentration by volume for 24 h. At these concentrations, only slight growth inhibitory effect was observed in 293 human kidney fibroblast cells and in three non-malignant bone marrows. Intracellular nitro blue tetrazolium (NBT) reduction assay showed that both MDA-MB231 breast cancer and K-562 CML cells had about 30% reduction of intracellular NBT after incubation with 30% of EM-X2. Increased activity of superoxide dismutase (SOD) could be detected from both MDA-MB231 and K-562 cell lines after incubating with 30% of EM-X2. Taken together, our data suggested that EM-X could inhibit growth and reduce the regeneration potential of cancer cells, possibly through its antioxidation activity.

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November 2004
Volume 14 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chui CH, Cheng GY, Ke B, Lau FY, Wong RS, Kok SH, Fatima S, Cheung F, Cheng CH, Chan AS, Chan AS, et al: Growth inhibitory potential of effective microorganism fermentation extract (EM-X) on cancer cells. Int J Mol Med 14: 925-929, 2004.
APA
Chui, C.H., Cheng, G.Y., Ke, B., Lau, F.Y., Wong, R.S., Kok, S.H. ... Tang, J.C. (2004). Growth inhibitory potential of effective microorganism fermentation extract (EM-X) on cancer cells. International Journal of Molecular Medicine, 14, 925-929. https://doi.org/10.3892/ijmm.14.5.925
MLA
Chui, C. H., Cheng, G. Y., Ke, B., Lau, F. Y., Wong, R. S., Kok, S. H., Fatima, S., Cheung, F., Cheng, C. H., Chan, A. S., Tang, J. C."Growth inhibitory potential of effective microorganism fermentation extract (EM-X) on cancer cells". International Journal of Molecular Medicine 14.5 (2004): 925-929.
Chicago
Chui, C. H., Cheng, G. Y., Ke, B., Lau, F. Y., Wong, R. S., Kok, S. H., Fatima, S., Cheung, F., Cheng, C. H., Chan, A. S., Tang, J. C."Growth inhibitory potential of effective microorganism fermentation extract (EM-X) on cancer cells". International Journal of Molecular Medicine 14, no. 5 (2004): 925-929. https://doi.org/10.3892/ijmm.14.5.925