Heterogeneous expression of DNA-dependent protein kinase in esophageal cancer and normal epithelium

  • Authors:
    • Norio Tonotsuka
    • Yoshio Hosoi
    • Shukichi Miyazaki
    • Go Miyata
    • Ko Sugawara
    • Takahiro Mori
    • Noriaki Ouchi
    • Susumu Satomi
    • Yoshihisa Matsumoto
    • Keiichi Nakagawa
    • Kiyoshi Miyagawa
    • Tetsuya Ono
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.441
  • Pages: 441-447
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Abstract

Esophageal cancer tissues and adjacent normal mucosae in 13 patients with primary esophageal cancer were examined for quantitative differences in DNA-dependent protein kinase (DNA-PK) activity and for expressions of Ku70, Ku80 and DNA-PKcs proteins by Western blotting and immunohistochemistry. The tumor tissues showed higher DNA-PK activity than the normal mucosae. Protein levels of Ku70, Ku80 and DNA-PKcs correlated with DNA-PK activities in the tumor tissues. Immunohistochemical analysis revealed that Ku70, Ku80 and DNA-PKcs located predominantly in the nuclei in both the tumor tissues and normal mucosae. In the normal epithelium, Ku70, Ku80 and DNA-PKcs were expressed only in the nuclei of the basal cell layers and not in those of the lumenal cell layers. In the tumor tissues, the expressions of DNA-PK proteins showed intratumoral heterogeneity. The different portions in the same tumor showed different expression levels of DNA-PK proteins, and even each tumor cell showed different expression levels. These results suggest that cell differentiation and tumor progression affect cellular DNA-PK protein levels and its activity. Furthermore, the intratumoral heterogeneity of DNA-PK protein expression in esophageal cancer cells/ tissues also suggests the difficulty in prediction of radio- or chemo-sensitivity of the tumor through estimation of DNA-PK activity/protein levels in tumor specimens.

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September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tonotsuka N, Hosoi Y, Miyazaki S, Miyata G, Sugawara K, Mori T, Ouchi N, Satomi S, Matsumoto Y, Nakagawa K, Nakagawa K, et al: Heterogeneous expression of DNA-dependent protein kinase in esophageal cancer and normal epithelium. Int J Mol Med 18: 441-447, 2006.
APA
Tonotsuka, N., Hosoi, Y., Miyazaki, S., Miyata, G., Sugawara, K., Mori, T. ... Ono, T. (2006). Heterogeneous expression of DNA-dependent protein kinase in esophageal cancer and normal epithelium. International Journal of Molecular Medicine, 18, 441-447. https://doi.org/10.3892/ijmm.18.3.441
MLA
Tonotsuka, N., Hosoi, Y., Miyazaki, S., Miyata, G., Sugawara, K., Mori, T., Ouchi, N., Satomi, S., Matsumoto, Y., Nakagawa, K., Miyagawa, K., Ono, T."Heterogeneous expression of DNA-dependent protein kinase in esophageal cancer and normal epithelium". International Journal of Molecular Medicine 18.3 (2006): 441-447.
Chicago
Tonotsuka, N., Hosoi, Y., Miyazaki, S., Miyata, G., Sugawara, K., Mori, T., Ouchi, N., Satomi, S., Matsumoto, Y., Nakagawa, K., Miyagawa, K., Ono, T."Heterogeneous expression of DNA-dependent protein kinase in esophageal cancer and normal epithelium". International Journal of Molecular Medicine 18, no. 3 (2006): 441-447. https://doi.org/10.3892/ijmm.18.3.441