Glucosamine suppresses interleukin-8 production and ICAM-1 expression by TNF-α-stimulated human colonic epithelial HT-29 cells

  • Authors:
    • Shin Yomogida
    • Jian Hua
    • Koji Sakamoto
    • Isao Nagaoka
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/ijmm_00000010
  • Pages: 205-211
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Abstract

Intestinal epithelial cells play an important role in the mucosal immune reaction in inflammatory bowel diseases via the production and expression of chemokines and adhesion molecules, such as interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1), which are involved in the neutrophil infiltration and tissue damage in the inflamed colon. Notably, glucosamine, a naturally-occurring amino monosaccharide, has been shown to exhibit an anti-inflammatory action by inhibiting neutrophil functions. In the present study, to evaluate the anti-inflammatory action of glucosamine on intestinal epithelial cells, we examined the effects of glucosamine on the activation of a human colonic epithelial cell line HT-29. The results revealed that glucosamine suppressed the IL-8 production and ICAM-1 expression by TNF-α-activated HT-29 cells. Furthermore, glucosamine inhibited the TNF-α-induced phosphorylation of p38MAPK and NF-κB p65, and the nuclear translocation of NF-κB in the cells. Thus, glucosamine demonstrates inhibitory actions on the inflammatory and signaling molecules (IL-8, ICAM-1, p38MAPK and NF-κB) in intestinal epithelial cells. However, glucosamine did not essentially affect the binding of TNF-α to its receptor on HT-29 cells. Together, these observations suggest that glucosamine may have the potential to exhibit an anti-inflammatory action on intestinal epithelial cells, by possibly interfering with the activation signaling downstream of the ligand/receptor binding.

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August 2008
Volume 22 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yomogida S, Hua J, Sakamoto K and Nagaoka I: Glucosamine suppresses interleukin-8 production and ICAM-1 expression by TNF-α-stimulated human colonic epithelial HT-29 cells. Int J Mol Med 22: 205-211, 2008.
APA
Yomogida, S., Hua, J., Sakamoto, K., & Nagaoka, I. (2008). Glucosamine suppresses interleukin-8 production and ICAM-1 expression by TNF-α-stimulated human colonic epithelial HT-29 cells. International Journal of Molecular Medicine, 22, 205-211. https://doi.org/10.3892/ijmm_00000010
MLA
Yomogida, S., Hua, J., Sakamoto, K., Nagaoka, I."Glucosamine suppresses interleukin-8 production and ICAM-1 expression by TNF-α-stimulated human colonic epithelial HT-29 cells". International Journal of Molecular Medicine 22.2 (2008): 205-211.
Chicago
Yomogida, S., Hua, J., Sakamoto, K., Nagaoka, I."Glucosamine suppresses interleukin-8 production and ICAM-1 expression by TNF-α-stimulated human colonic epithelial HT-29 cells". International Journal of Molecular Medicine 22, no. 2 (2008): 205-211. https://doi.org/10.3892/ijmm_00000010