Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.

  • Authors:
    • Y Ma
    • B Gunn
    • V S Byers
    • R W Baldwin
    • M R Price
  • View Affiliations

  • Published online on: June 1, 1999     https://doi.org/10.3892/ijmm.3.6.621
  • Pages: 621-625
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Abstract

In an analysis of murine immune responses to the dust mite allergen Der p 1, treatment with purified allergen induced a significant increase in the level of circulating IgE immunoglobulin (from less than 100 ng/ml in normal mice to 1,350 ng/ml in mice receiving the allergen). Even so, specific IgE antibodies binding to purified Der p 1 were not detected in a conventional ELISA, and the major response appeared to be the induction of high titre IgG antibodies. Specific circulating murine IgE antibodies were however detected using the following assay format: murine IgE was captured to anti-murine IgE antibody coated wells; Der p 1 was added and bound by immobilized anti-Der p 1 IgE antibodies; the captured Der p 1 was then detected by the addition of monoclonal IgG antibodies against Der p 1 and these antibodies were measured by the addition of anti-murine IgG antibody-enzyme conjugate with which colour development is produced after substrate addition. This assay establishes a procedure to measure circulating anti-Der p 1 IgE antibodies which are present together with competing high titre IgG anti-Der p 1 antibodies.

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Jun 1999
Volume 3 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ma Y, Gunn B, Byers V, Baldwin R and Price M: Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.. Int J Mol Med 3: 621-625, 1999.
APA
Ma, Y., Gunn, B., Byers, V., Baldwin, R., & Price, M. (1999). Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.. International Journal of Molecular Medicine, 3, 621-625. https://doi.org/10.3892/ijmm.3.6.621
MLA
Ma, Y., Gunn, B., Byers, V., Baldwin, R., Price, M."Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.". International Journal of Molecular Medicine 3.6 (1999): 621-625.
Chicago
Ma, Y., Gunn, B., Byers, V., Baldwin, R., Price, M."Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.". International Journal of Molecular Medicine 3, no. 6 (1999): 621-625. https://doi.org/10.3892/ijmm.3.6.621