Significant correlation between the acceleration of platelet aggregation and phosphorylation of HSP27 at Ser-78 in diabetic patients

  • Authors:
    • Haruhiko Tokuda
    • Kenji Kato
    • Senji Kasahara
    • Rie Matsushima-Nishiwaki
    • Takahiko Mizuno
    • Seiko Sakakibara
    • Osamu Kozawa
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  • Published online on: October 1, 2012     https://doi.org/10.3892/ijmm.2012.1146
  • Pages: 1387-1395
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Abstract

To clarify the mechanism underlying a high risk of thrombotic complications in diabetic patients, we investigated the relationship between HSP27 phosphorylation and the platelet activation induced by adenosine diphosphate (ADP) in diabetic patients. Platelet-rich plasma was prepared from the blood of type 2 diabetes mellitus (DM) patients. By measuring the dose response of platelet aggregation to ADP, an individual ED50 was determined. Based on the normal range identified in non-DM controls, the subjects were divided into a hyper-aggregate (Group 1) and a normo- or hypo-aggregate group (Group 2). The protein phosphorylation was analyzed by western blotting. The release of PDGF-AB and sCD40 ligand (sCD40L) was measured by ELISA. In both groups, ADP induced HSP27 phosphorylation at Ser-78 and Ser-82. The phosphorylation at Ser-78 and the release of both PDGF-AB and sCD40L induced by a low dose of ADP (1 µM) in Group 1 were significantly higher than these values in Group 2. There was a significant relationship between the ADP-induced HSP27 phosphorylation level at Ser-78 and the ADP ED50 value of platelet aggregation. The ADP (1 µM)-induced phosphorylation of HSP at Ser-78 observed in the platelets from Group 1 was inhibited by PD98059 or SB203580. The use of aspirin ameliorated the accelerated microaggregation of platelets in Group 1, and the low-dose ADP-induced phosphorylation of HSP27 at Ser-78 was no longer observed. These results strongly suggest that the phosphorylation of HSP27 at Ser-78 is correlated with the acceleration of platelet aggregation induced by ADP in type 2 DM patients.
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December 2012
Volume 30 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tokuda H, Kato K, Kasahara S, Matsushima-Nishiwaki R, Mizuno T, Sakakibara S and Kozawa O: Significant correlation between the acceleration of platelet aggregation and phosphorylation of HSP27 at Ser-78 in diabetic patients. Int J Mol Med 30: 1387-1395, 2012.
APA
Tokuda, H., Kato, K., Kasahara, S., Matsushima-Nishiwaki, R., Mizuno, T., Sakakibara, S., & Kozawa, O. (2012). Significant correlation between the acceleration of platelet aggregation and phosphorylation of HSP27 at Ser-78 in diabetic patients. International Journal of Molecular Medicine, 30, 1387-1395. https://doi.org/10.3892/ijmm.2012.1146
MLA
Tokuda, H., Kato, K., Kasahara, S., Matsushima-Nishiwaki, R., Mizuno, T., Sakakibara, S., Kozawa, O."Significant correlation between the acceleration of platelet aggregation and phosphorylation of HSP27 at Ser-78 in diabetic patients". International Journal of Molecular Medicine 30.6 (2012): 1387-1395.
Chicago
Tokuda, H., Kato, K., Kasahara, S., Matsushima-Nishiwaki, R., Mizuno, T., Sakakibara, S., Kozawa, O."Significant correlation between the acceleration of platelet aggregation and phosphorylation of HSP27 at Ser-78 in diabetic patients". International Journal of Molecular Medicine 30, no. 6 (2012): 1387-1395. https://doi.org/10.3892/ijmm.2012.1146