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Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion

  • Authors:
    • Yiguan Xu
    • Xuerui Tan
    • Dongming Wang
    • Wei Wang
    • Yuguang Li
    • Min Wu
    • Songming Chen
    • Yinge Wu
    • Chunjiang Tan

  • View Affiliations

  • Published online on: March 24, 2015     https://doi.org/10.3892/ijmm.2015.2154
  • Pages: 1501-1510

  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Survivin is essential to angiogenesis and revascularization, but its role in coronary collateral formation remains unclear. The role of survivin in peripheral blood mononuclear cells (PBMCs) of coronary chronic total occlusion (CTO) patients was investigated. Coronary CTO patients (n=46; mean age 60.1±8.5, male 54.3%) (CTO group) and normal control patients (n=18; mean age 58.0±10.0, male 55.6%) underwent angiographic collateral vessel grading by Rentrop classification (C0 - C3) and provided peripheral blood between June 2006 and February 2007. Rat hind limb ischemia models were constructed using four equal groups of Sprague-Dawley rats (n=36): normal control, sham operation, operation and granulocyte macrophage colony‑stimulating factor (GM-CSF). PBMC numbers and characteristics, collateral vessels, survivin, CD4, CD8, CD44, vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) expression were determined using RT-PCR, flow cytometry, immunocytochemistry and western blot analysis. PBMC survivin mRNA and protein expression levels were higher in patients with good collateral circulation (C2 + C3) than in patients with no collateral flow (C0) (all P<0.05). Survivin single‑positive and survivin and CD8, VEGF and ICAM-1 double‑positive percentages were elevated in patients with good collateral circulation compared to those with normal and no collateral flow (all P<0.05), consistent with the rat model results, wherein higher survivin levels produced significantly larger and more visible collateral vessels. In conclusion, elevated survivin expression in PBMCs, particularly survivin and CD8, VEGF, and ICAM-1 double‑positive PBMCs, may be crucial for good collateral formation in patients with coronary CTO, as confirmed by assessment of a rat model.
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June-2015
Volume 35 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y and Tan C: Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion. Int J Mol Med 35: 1501-1510, 2015
APA
Xu, Y., Tan, X., Wang, D., Wang, W., Li, Y., Wu, M. ... Tan, C. (2015). Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion. International Journal of Molecular Medicine, 35, 1501-1510. https://doi.org/10.3892/ijmm.2015.2154
MLA
Xu, Y., Tan, X., Wang, D., Wang, W., Li, Y., Wu, M., Chen, S., Wu, Y., Tan, C."Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion". International Journal of Molecular Medicine 35.6 (2015): 1501-1510.
Chicago
Xu, Y., Tan, X., Wang, D., Wang, W., Li, Y., Wu, M., Chen, S., Wu, Y., Tan, C."Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion". International Journal of Molecular Medicine 35, no. 6 (2015): 1501-1510. https://doi.org/10.3892/ijmm.2015.2154