Open Access

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo

  • Authors:
    • Dan Wang
    • Xiaoming Ding
    • Wujun Xue
    • Jin Zheng
    • Xiaohui Tian
    • Yang Li
    • Xiaohong Wang
    • Huanjin Song
    • Hua Liu
    • Xiaohui Luo
  • View Affiliations

  • Published online on: Tuesday, November 29, 2016
  • Pages:167-173 DOI: 10.3892/ijmm.2016.2814
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It is unknown whether a scaffold containing both small intestinal submucosa (SIS) and mesenchymal stem cells (MSCs) for transplantation may improve pancreatic islet function and survival. In this study, we examined the effects of a SIS-MSC scaffold on islet function and survival in vitro and in vivo. MSCs and pancreatic islets were isolated from Sprague-Dawley rats, and SIS was isolated from Bamei pigs. The islets were apportioned among 3 experimental groups as follows: SIS-islets, SIS-MSC-islets and control-islets. In vitro, islet function was measured by a glucose-stimulated insulin secretion test; cytokines in cultured supernatants were assessed by enzyme-linked immunosorbent assay; and gene expression was analyzed by reverse transcription-quantitative PCR. In vivo, islet transplantation was performed in rats, and graft function and survival were monitored by measuring the blood glucose levels. In vitro, the SIS-MSC scaffold was associated with improved islet viability and enhanced insulin secretion compared with the controls, as well as with the increased the expression of insulin 1 (Ins1), pancreatic and duodenal homeobox 1 (Pdx1), platelet endothelial cell adhesion molecule 1 [Pecam1; also known as cluster of differentiation 31 (CD31)] and vascular endothelial growth factor A (Vegfa) in the islets, increased growth factor secretion, and decreased tumor necrosis factor (TNF) secretion. In vivo, the SIS-MSC scaffold was associated with improved islet function and graft survival compared with the SIS and control groups. On the whole, our findings demonstrate that the SIS-MSC scaffold significantly improved pancreatic islet function and survival in vitro and in vivo. This improvement may be associated with the upregulation of insulin expression, the improvement of islet microcirculation and the secretion of cytokines.

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January 2017
Volume 39 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Wang, D., Ding, X., Xue, W., Zheng, J., Tian, X., Li, Y. ... Luo, X. (2017). A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo. International Journal of Molecular Medicine, 39, 167-173. http://dx.doi.org/10.3892/ijmm.2016.2814
MLA
Wang, D., Ding, X., Xue, W., Zheng, J., Tian, X., Li, Y., Wang, X., Song, H., Liu, H., Luo, X."A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo". International Journal of Molecular Medicine 39.1 (2017): 167-173.
Chicago
Wang, D., Ding, X., Xue, W., Zheng, J., Tian, X., Li, Y., Wang, X., Song, H., Liu, H., Luo, X."A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo". International Journal of Molecular Medicine 39, no. 1 (2017): 167-173. http://dx.doi.org/10.3892/ijmm.2016.2814