Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells

Yin,Xiao; Zeng,Zhaobin; Xing,Jinliang; Zhang,Ansheng; Jiang,Wenkai; Wang,Wei; Sun,Hantang; Ni,Longxing

doi:10.3892/ijmm.2017.3254

Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells

Authors:
- Xiao Yin
- Zhaobin Zeng
- Jinliang Xing
- Ansheng Zhang
- Wenkai Jiang
- Wei Wang
- Hantang Sun
- Longxing Ni
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Affiliations: State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110015, P.R. China, Preclinical Medical Teaching Experimental Center, School of Basic Medical Sciences, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
Published online on: November 13, 2017 https://doi.org/10.3892/ijmm.2017.3254
Pages: 331-339
Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Substantial evidence has indicated that Notch and bone morphogenetic protein (BMP) signaling may regulate odontoblastic differentiation. Hairy/enhancer‑of‑split related with YRPW motif 1 (Hey1), a downstream target gene of Notch and BMP signaling, is expressed in dental pulp tissues and has been demonstrated to be responsible for osteoblast mineralization. The aim of this study was to investigate the effects of Hey1 on odontoblast differentiation. The results of the study demonstrated that Hey1 expression in odontoblast‑lineage cells (OLCs) was upregulated by stimulation of osteoblastic/odontoblastic differentiation medium containing ascorbic acid, β‑glycerol phosphate and dexamethasone. Furthermore, stable Hey1‑overexpressing cells expressed higher levels of dentin sialophosphoprotein (DSPP) and exhibited higher mineralization capabilities following stimulation by differentiation medium. Furthermore, RNA interference‑mediated knockdown of Hey1 downregulated the expression levels of DSPP in OLCs stimulated by differentiation medium. Taken together, the findings indicate that Hey1 may be a positive regulator of odontoblastic differentiation. The present study broadens the understanding of odontoblast differentiation and biomineralization.

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