Paediatric Virology and its interaction between basic science and clinical practice (Review)

Mammas,Ioannis  N.; Greenough,Anne; Theodoridou,Maria; Kramvis,Anna; Rusan,Maria; Melidou,Angeliki; Korovessi,Paraskevi; Papaioannou,Georgia; Papatheodoropoulou,Alexia; Koutsaftiki,Chryssie; Liston,Maria; Sourvinos,George; Spandidos,Demetrios   A.

doi:10.3892/ijmm.2018.3364

Paediatric Virology and its interaction between basic science and clinical practice (Review)

Authors:
- Ioannis N. Mammas
- Anne Greenough
- Maria Theodoridou
- Anna Kramvis
- Maria Rusan
- Angeliki Melidou
- Paraskevi Korovessi
- Georgia Papaioannou
- Alexia Papatheodoropoulou
- Chryssie Koutsaftiki
- Maria Liston
- George Sourvinos
- Demetrios A. Spandidos
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Affiliations: Department of Clinical Virology, School of Medicine, University of Crete, Heraklion 71003, Greece, Division of Asthma, Allergy and Lung Biology, King's College London, London SE5 9RS, UK, 1st Department of Paediatrics, ‘Aghia Sophia’ Children's Hospital, University of Athens School of Medicine, Athens 11527, Greece, Hepatitis Virus Diversity Research Unit, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa, Department of Medical Oncology, Dana‑Farber Cancer Institute, Boston, MA 02115, USA, 2nd Laboratory of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece, Department of Paediatrics, ‘Penteli’ Children's Hospital, Penteli 1523, Greece, Department of Paediatric Radiology, ‘Mitera’ Children's Hospital, Athens 15123, Greece, Paediatric Intensive Care Unit (PICU), ‘Aglaia Kyriakou’ Children's Hospital, Athens 11527, Greece, Paediatric Intensive Care Unit (PICU), ‘Penteli’ Children's Hospital, Penteli 15236, Greece, Department of Anthropology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
Published online on: January 4, 2018 https://doi.org/10.3892/ijmm.2018.3364
Pages: 1165-1176
Copyright: © Mammas et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The 3rd Workshop on Paediatric Virology, which took place on October 7th, 2017 in Athens, Greece, highlighted the role of breast feeding in the prevention of viral infections during the first years of life. Moreover, it focused on the long-term outcomes of respiratory syncytial virus and rhinovirus infections in prematurely born infants and emphasised the necessity for the development of relevant preventative strategies. Other topics that were covered included the vaccination policy in relation to the migration crisis, mother‑to‑child transmission of hepatitis B and C viruses, vaccination against human papilloma viruses in boys and advances on intranasal live‑attenuated vaccination against influenza. Emphasis was also given to the role of probiotics in the management of viral infections in childhood, the potential association between viral infections and the pathogenesis of asthma, fetal and neonatal brain imaging and the paediatric intensive care of children with central nervous system viral infections. Moreover, an interesting overview of the viral causes of perinatal mortality in ancient Greece was given, where recent archaeological findings from the Athenian Agora's bone well were presented. Finally, different continuing medical educational options in Paediatric Virology were analysed and evaluated. The present review provides an update of the key topics discussed during the workshop.

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1	Greenough A, Osborne J and Sutherland S: Congenital, Perinatal, and Neonatal Infections. Churchill Livingstone; Edinburgh: 1992
2	Shulman ST: The history of pediatric infectious diseases. Pediatr Res. 55:163–176. 2004. View Article : Google Scholar
3	Starr M: Paediatric infectious diseases: The last 50 years. J Paediatr Child Health. 51:12–15. 2015. View Article : Google Scholar : PubMed/NCBI
4	Mammas IN, Greenough A, Theodoridou M, Kramvis A, Christaki I, Koutsaftiki C, Koutsaki M, Portaliou DM, Kostagianni G, Panagopoulou P, Sourvinos G and Spandidos DA: Current views and advances on Paediatric Virology: An update for paediatric trainees. Exp Ther Med. 11:6–14. 2016. View Article : Google Scholar : PubMed/NCBI
5	Mammas IN, Greenough A, Theodoridou M and Spandidos DA: Paediatric Virology: A new paediatric subspecialty? A proposal at the Workshop on Paediatric Virology, Athens, October 10,2015. Exp Ther Med. 11:3–5. 2016. View Article : Google Scholar : PubMed/NCBI
6	zur Hausen H: Paediatric Virology and Oncology: Virus persistence and the important first years of life. Int J Mol Med. 40(Suppl 41)2017.
7	zur Hausen H, Bund T and de Villiers EM: Infectious agents in bovine red meat and milk and their potential role in cancer and other chronic diseases. Curr Top Microbiol Immunol. 407:83–116. 2017.PubMed/NCBI
8	Greenough A, MacBean V, Lunt A, Drysdale SB, Rafferty GF and Peacock JL: The long term impact of viral infections on prematurely born infants. Int J Mol Med. 40(Suppl 41)2017.
9	Broughton S, Bhat R, Roberts A, Zuckerman M, Rafferty G and Greenough A: Diminished lung function, RSV infection, and respiratory morbidity in prematurely born infants. Arch Dis Child. 91:26–30. 2006. View Article : Google Scholar
10	Drysdale SB, Wilson T, Alcazar M, Broughton S, Zuckerman M, Smith M, Rafferty GF, Johnston SL and Greenough A: Lung function prior to viral lower respiratory tract infections in prematurely born infants. Thorax. 66:468–473. 2011. View Article : Google Scholar : PubMed/NCBI
11	Drysdale SB, Prendergast M, Alcazar M, Wilson T, Smith M, Zuckerman M, Broughton S, Rafferty GF, Johnston SL, Hodemaekers HM, et al: Genetic predisposition of RSV infection-related respiratory morbidity in preterm infants. Eur J Pediatr. 173:905–912. 2014. View Article : Google Scholar : PubMed/NCBI
12	Drysdale SB, Lo J, Prendergast M, Alcazar M, Wilson T, Zuckerman M, Smith M, Broughton S, Rafferty GF, Peacock JL, et al: Lung function of preterm infants before and after viral infections. Eur J Pediatr. 173:1497–1504. 2014. View Article : Google Scholar : PubMed/NCBI
13	Broughton S, Roberts A, Fox G, Pollina E, Zuckerman M, Chaudhry S and Greenough A: Prospective study of healthcare utilisation and respiratory morbidity due to RSV infection in prematurely born infants. Thorax. 60:1039–1044. 2005. View Article : Google Scholar : PubMed/NCBI
14	Greenough A, Alexander J, Boit P, Boorman J, Burgess S, Burke A, Chetcuti PA, Cliff I, Lenney W, Lytle T, et al: School age outcome of hospitalisation with respiratory syncytial virus infection of prematurely born infants. Thorax. 64:490–495. 2009. View Article : Google Scholar : PubMed/NCBI
15	Chidekel AS, Rosen CL and Bazzy AR: Rhinovirus infection associated with serious lower respiratory illness in patients with bronchopulmonary dysplasia. Pediatr Infect Dis J. 16:43–47. 1997. View Article : Google Scholar : PubMed/NCBI
16	Drysdale SB, Alcazar-Paris M, Wilson T, Smith M, Zuckerman M, Broughton S, Rafferty GF, Peacock JL, Johnston SL and Greenough A: Rhinovirus infection and healthcare utilisation in prematurely born infants. Eur Respir J. 42:1029–1036. 2013. View Article : Google Scholar : PubMed/NCBI
17	MacBean V, Lunt A, Drysdale S, Rafferty GF and Greenough A: Predicting healthcare outcomes in prematurely born infants using cluster analysis. Arch Dis Child. 102:A190–A191. 2017.
18	MacBean V, Yarzi MN, Drysdale S, Rafferty GF and Greenough A: Impact of respiratory viral infections on school age outcomes in prematurely born children. Am J Respir Crit Care Med. 195:A21692017.
19	Theodoridou M: Immigration crisis and vaccination policy. Int J Mol Med. 40:S412017.
20	United Nations High Commissioner for Refugees (UNHCR): The sea route to Europe: The Mediterranean passage in the age of refugees. 2015, http://www.unhcr.org/5592bd059.pdf.
21	Medecins Sans Frontieres (MSF): EU Migration Crisis Update - February 2016. 2016, http://www.msf.org/en/article/eu-migration-crisis-update-february-2016.
22	International Organization for Migration (IOM): IOM: 170.553 migrants/refugees have arrived in Greece in 2016. 2016, https://greece.iom.int/en/news/iom-170553-migrantsrefugees-have-arrived-greece-2016.
23	REACH: Situation overview: European migration crisis, Western Balkans. December. 2015, https://www.impact-repository.org/document/reach/a43ee2d2/reach_eu_situation_overview_european_migration_trends_december_2015_5.pdf.
24	Mipatrini D, Stefanelli P, Severoni S and Rezza G: Vaccinations in migrants and refugees: A challenge for European health systems. A systematic review of current scientific evidence. Pathog Glob Health. 111:59–68. 2017. View Article : Google Scholar : PubMed/NCBI
25	World Health Organization (WHO). Refugee and migrant health. 2017, http://www.who.int/migrants/en/.
26	Hellenic Ministry of Health. Report. Vaccination on Points of care of refugees/migrants. February. 2017, http://www.moh.gov.gr/articles/ministry/grafeio-typoy/press-releases/4489-ekthesh-emboliastikhs-kalypshs-paidiwn-metanastwn-kai-prosfygwn.
27	Giambi C, Del Manso M, Dente MG, Napoli C, Montaño-Remacha C, Riccardo F and Declich S; Network For The Control Of Cross-Border Health Threats In The Mediterranean Basin And Black Sea For The ProVacMed Project: Immunization strategies targeting newly arrived migrants in non-EU countries of the Mediterranean Basin and Black Sea. Int J Environ Res Public Health. 14:142017. View Article : Google Scholar
28	World Health Organization (WHO): World Health Organization Global Hepatitis Report 2017. 2017, http://www.who.int/hepatitis/publications/global-hepatitis-report2017/en/.
29	Kramvis A: Mother to child transmission of hepatitis B and C viruses. Int J Mol Med. 40:S392017.
30	Shimakawa Y, Lemoine M, Bottomley C, Njai HF, Ndow G, Jatta A, Tamba S, Bojang L, Taal M, Nyan O, et al: Birth order and risk of hepatocellular carcinoma in chronic carriers of hepatitis B virus: A case-control study in The Gambia. Liver Int. 35:2318–2326. 2015. View Article : Google Scholar : PubMed/NCBI
31	Kramvis A: The clinical implications of hepatitis B virus genotypes and HBeAg in pediatrics. Rev Med Virol. 26:285–303. 2016. View Article : Google Scholar : PubMed/NCBI
32	Tovo PA, Calitri C, Scolfaro C, Gabiano C and Garazzino S: Vertically acquired hepatitis C virus infection: Correlates of transmission and disease progression. World J Gastroenterol. 22:1382–1392. 2016. View Article : Google Scholar : PubMed/NCBI
33	World Health Organization (WHO). Global Health Sector Strategy on viral hepatitis 2016–2021. 2016, http://www.who.int/hepatitis/strategy2016-2021/ghss-hep/en/.
34	Schillie S, Walker T, Veselsky S, Crowley S, Dusek C, Lazaroff J, Morris SA, Onye K, Ko S, Fenlon N, et al: Outcomes of infants born to women infected with hepatitis B. Pediatrics. 135:e1141–e1147. 2015. View Article : Google Scholar : PubMed/NCBI
35	Brown RS Jr, McMahon BJ, Lok AS, Wong JB, Ahmed AT, Mouchli MA, Wang Z, Prokop LJ, Murad MH and Mohammed K: Antiviral therapy in chronic hepatitis B viral infection during pregnancy: A systematic review and meta-analysis. Hepatology. 63:319–333. 2016. View Article : Google Scholar
36	Pan CQ, Zou HB, Chen Y, Zhang X, Zhang H, Li J and Duan Z: Cesarean section reduces perinatal transmission of hepatitis B virus infection from hepatitis B surface antigen-positive women to their infants. Clin Gastroenterol Hepatol. 11:1349–1355. 2013. View Article : Google Scholar : PubMed/NCBI
37	Chen X, Chen J, Wen J, Xu C, Zhang S, Zhou YH and Hu Y: Breastfeeding is not a risk factor for mother-to-child transmission of hepatitis B virus. PLoS One. 8:e553032013. View Article : Google Scholar : PubMed/NCBI
38	Benova L, Mohamoud YA, Calvert C and Abu-Raddad LJ: Vertical transmission of hepatitis C virus: Systematic review and meta-analysis. Clin Infect Dis. 59:765–773. 2014. View Article : Google Scholar : PubMed/NCBI
39	Elrazek A, Amer M, El-Hawary B, Salah A, Bhagavathula AS, Alboraie M and Saab S: Prediction of HCV vertical transmission: What factors should be optimized using data mining computational analysis. Liver Int. 37:529–533. 2017. View Article : Google Scholar
40	Rusan M: Human papillomavirus infections in children: Should we be vaccinating earlier? Int J Mol Med. 40:S402017.
41	Doorbar J, Quint W, Banks L, Bravo IG, Stoler M, Broker TR and Stanley MA: The biology and life-cycle of human papillomaviruses. Vaccine. 30(Suppl 5): F55–F70. 2012. View Article : Google Scholar : PubMed/NCBI
42	Kajitani N, Satsuka A, Kawate A and Sakai H: Productive lifecycle of human papillomaviruses that depends upon squamous epithelial differentiation. Front Microbiol. 3:1522012. View Article : Google Scholar : PubMed/NCBI
43	Lehoux M, D’Abramo CM and Archambault J: Molecular mechanisms of human papillomavirus-induced carcinogenesis. Public Health Genomics. 12:268–280. 2009. View Article : Google Scholar : PubMed/NCBI
44	Trottier H and Burchell AN: Epidemiology of mucosal human papillomavirus infection and associated diseases. Public Health Genomics. 12:291–307. 2009. View Article : Google Scholar : PubMed/NCBI
45	Forman D, de Martel C, Lacey CJ, Soerjomataram I, Lortet-Tieulent J, Bruni L, Vignat J, Ferlay J, Bray F, Plummer M, et al: global burden of human papillomavirus and related diseases. Vaccine. 30(Suppl 5): F12–F23. 2012. View Article : Google Scholar : PubMed/NCBI
46	Syrjänen S: Current concepts on human papillomavirus infections in children. APMIS. 118:494–509. 2010. View Article : Google Scholar : PubMed/NCBI
47	Mammas IN, Sourvinos G and Spandidos DA: Human papilloma virus (HPV) infection in children and adolescents. Eur J Pediatr. 168:267–273. 2009. View Article : Google Scholar
48	Tiggelaar SM, Lin MJ, Viscidi RP, Ji J and Smith JS: Age-specific human papillomavirus antibody and deoxyribonucleic acid prevalence: A global review. J Adolesc Health. 50:110–131. 2012. View Article : Google Scholar : PubMed/NCBI
49	Silverberg MJ, Thorsen P, Lindeberg H, Grant LA and Shah KV: Condyloma in pregnancy is strongly predictive of juvenile-onset recurrent respiratory papillomatosis. Obstet Gynecol. 101:645–652. 2003.PubMed/NCBI
50	Michels KB and zur Hausen H: HPV vaccine for all. Lancet. 374:268–270. 2009. View Article : Google Scholar : PubMed/NCBI
51	Ontario: Getting the HPV vaccine. Government of Ontario; 2017, http://www.health.gov.on.ca/en/ms/hpv/.
52	Centers for Disease Control and Prevention (CDC): HPV Vaccines: Vaccinating Your Preteen or Teen. 2016, https://www.cdc.gov/hpv/parents/vaccine.html.
53	Paul KT: ‘Saving lives’: Adapting and adopting Human Papilloma Virus (HPV) vaccination in Austria. Soc Sci Med. 153:193–200. 2016. View Article : Google Scholar : PubMed/NCBI
54	World Health Organization (WHO): Human papillomavirus vaccines: WHO position paper, May 2017-Recommendations. Vaccine. 2017.In press.
55	Palefsky JM, Giuliano AR, Goldstone S, Moreira ED Jr, Aranda C, Jessen H, Hillman R, Ferris D, Coutlee F, Stoler MH, et al: HPV vaccine against anal HPV infection and anal intraepithelial neoplasia. N Engl J Med. 365:1576–1585. 2011. View Article : Google Scholar : PubMed/NCBI
56	Anic GM and Giuliano AR: Genital HPV infection and related lesions in men. Prev Med. 53(Suppl 1): S36–S41. 2011. View Article : Google Scholar : PubMed/NCBI
57	Herrero R, Quint W, Hildesheim A, Gonzalez P, Struijk L, Katki HA, Porras C, Schiffman M, Rodriguez AC, Solomon D, et al CVT Vaccine Group: Reduced prevalence of oral human papillomavirus (HPV) 4 years after bivalent HPV vaccination in a randomized clinical trial in Costa Rica. PLoS One. 8:e683292013. View Article : Google Scholar : PubMed/NCBI
58	Drolet M, Bénard É, Boily MC, Ali H, Baandrup L, Bauer H, Beddows S, Brisson J, Brotherton JM, Cummings T, et al: Population-level impact and herd effects following human papillomavirus vaccination programmes: A systematic review and meta-analysis. Lancet Infect Dis. 15:565–580. 2015. View Article : Google Scholar : PubMed/NCBI
59	Vichnin M, Bonanni P, Klein NP, Garland SM, Block SL, Kjaer SK, Sings HL, Perez G, Haupt RM, Saah AJ, et al: An overview of quadrivalent human papillomavirus vaccine safety: 2006 to 2015. Pediatr Infect Dis J. 34:983–991. 2015. View Article : Google Scholar : PubMed/NCBI
60	Melidou A: Intranasal live-attenuated influenza vaccination in children: Evidence, limitations and proposals. Int J Mol Med. 40:S392017.
61	Rodgers L, Pabst LJ and Chaves SS: Increasing uptake of live attenuated influenza vaccine among children in the United States, 2008-2014. Vaccine. 33:22–24. 2015. View Article : Google Scholar
62	Centers for Disease Prevention and Control (CDC). Seasonal Influenza: Flu Basics. 2016, https://www.cdc.gov/flu/about/season/flu-season-2016-2017.htm.
63	Pebody R, Warburton F, Ellis J, Andrews N, Potts A, Cottrell S, Johnston J, Reynolds A, Gunson R, Thompson C, et al: Effectiveness of seasonal influenza vaccine for adults and children in preventing laboratory-confirmed influenza in primary care in the United Kingdom: 2015/16 end-of-season results. Euro Surveill. 21:212016.
64	Nohynek H, Baum U, Syrjänen R, Ikonen N, Sundman J and Jokinen J: Effectiveness of the live attenuated and the inactivated influenza vaccine in two-year-olds - a nationwide cohort study Finlandm influenza season 2015/16. Euro Surveill. 21:212016. View Article : Google Scholar
65	Kwong JC, Pereira JA, Quach S, Pellizzari R, Dusome E, Russell ML, Hamid JS, Feinberg Y, Winter AL, Gubbay JB, et al Public Health Agency of Canada/Canadian Institutes of Health Research Influenza Research Network (PCIRN) Program Delivery and Evaluation Group: Randomized evaluation of live attenuated vs. inactivated influenza vaccines in schools (RELATIVES) cluster randomized trial: Pilot results from a household surveillance study to assess direct and indirect protection from influenza vaccination. Vaccine. 33:4910–4915. 2015. View Article : Google Scholar : PubMed/NCBI
66	Caspard H, Coelingh KL, Mallory RM and Ambrose CS: Association of vaccine handling conditions with effectiveness of live attenuated influenza vaccine against H1N1pdm09 viruses in the United States. Vaccine. 34:5066–5072. 2016. View Article : Google Scholar : PubMed/NCBI
67	Caspard H, Heikkinen T, Belshe RB and Ambrose CS: A systematic review of the efficacy of live attenuated influenza vaccine upon revaccination of children. Hum Vaccin Immunother. 12:1721–1727. 2016.PubMed/NCBI
68	Leung VKY, Carolan LA, Worth LJ, Harper SA, Peck H, Tilmanis D, Laurie KL, Slavin MA and Sullivan SG: Influenza vaccination responses: Evaluating impact of repeat vaccination among health care workers. Vaccine. 35:2558–2568. 2017. View Article : Google Scholar : PubMed/NCBI
69	Simpson CR, Lone NI, Kavanagh K, Robertson C, McMenamin J, von Wissmann B, et al: Evaluating the effectiveness, impact and safety of live attenuated and seasonal inactivated influenza vaccination: protocol for the Seasonal Influenza Vaccination Effectiveness II (SIVE II) study. BMJ Open. 7:e0142002017.70. View Article : Google Scholar : PubMed/NCBI
70	Korovessi P: Probiotics and viral infections in childhood. Int J Mol Med. 40:S392017.
71	Food and Agriculture Organization of the United Nations, World Health Organization: Guidelines for the Evaluation of Probiotics in Food. In: Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food; London, Ontario, Canada. April 30 and May 1, 2002;
72	Ahrne S and Hagslatt ML: Effect of lactobacilli on paracellular permeability in the gut. Nutrients. 3:104–117. 2011. View Article : Google Scholar
73	Morais MB and Jacob CM: The role of probiotics and prebiotics in pediatric practice. J Pediatr (Rio J). 82(Suppl 5): S189–S197. 2006. View Article : Google Scholar
74	Guarino A, Lo Vecchio A and Canani RB: Probiotics as prevention and treatment for diarrhea. Curr Opin Gastroenterol. 25:18–23. 2009. View Article : Google Scholar
75	Szajewska H, Guarino A, Hojsak I, Indrio F, Kolacek S, Shamir R, Vandenplas Y and Weizman Z; European Society for Pediatric Gastroenterology, Hepatology and Nutrition: Use of probiotics for management of acute gastroenteritis: A position paper by the ESPGHAN Working Group for Probiotics and Prebiotics. J Pediatr Gastroenterol Nutr. 58:531–539. 2014. View Article : Google Scholar : PubMed/NCBI
76	Guarino A, Ashkenazi S, Gendrel D, Lo Vecchio A, Shamir R and Szajewska H; European Society for Pediatric Gastroenterology, Hepatology, and Nutrition; European Society for Pediatric Infectious Diseases: European Society for Pediatric Gastroenterology, Hepatology, and Nutrition/European Society for Pediatric Infectious Diseases evidence-based guidelines for the management of acute gastroenteritis in children in Europe; Update 2014. J Pediatr Gastroenterol Nutr. 59:132–152. 2014. View Article : Google Scholar : PubMed/NCBI
77	Hao Q, Lu Z, Dong BR, Huang CQ and Wu T: Probiotics for preventing acute upper respiratory tract infections. Cohrane Database Syst Rev. 9:CD0068952015. View Article : Google Scholar
78	Esposito S, Rigante D and Principi N: Do children’s upper respiratory tract infections benefit from probiotics? BMC Infect Dis. 14:1942014. View Article : Google Scholar
79	Allan GM and Arroll B: Prevention and treatment of the common cold: Making sense of the evidence. CMAJ. 186:190–199. 2014. View Article : Google Scholar : PubMed/NCBI
80	Amaral MA, Guedes GHBF, Epifanio M, Wagner MB, Jones MH and Mattiello R: Network meta-analysis of probiotics to prevent respiratory infections in children and adolescents. Pediatr Pulmonol. 52:833–843. 2017. View Article : Google Scholar : PubMed/NCBI
81	Papaioannou G: Foetal and neonatal brain imaging in viral infections. Int J Mol Med. 40:S392017.
82	Lo CP and Chen CY: Neuroimaging of viral infections in infants and young children. Neuroimaging Clin N Am. 18:119–132. 2008. View Article : Google Scholar : PubMed/NCBI
83	Shaw DWW and Cohen WA: Viral infections of the CNS in children: Imaging features. AJR Am J Roentgenol. 160:125–133. 1993. View Article : Google Scholar : PubMed/NCBI
84	Verstraelen H, Vanzieleghem B, Defoort P, Vanhaesebrouck P and Temmerman M: Prenatal ultrasound and magnetic resonance imaging in fetal varicella syndrome: Correlation with pathology findings. Prenat Diagn. 23:705–709. 2003. View Article : Google Scholar : PubMed/NCBI
85	Sanchez TR, Datlow MD and Nidecker AE: Diffuse periventricular calcification and brain atrophy: A case of neonatal central nervous system cytomegalovirus infection. Neuroradiol J. 29:314–316. 2016. View Article : Google Scholar : PubMed/NCBI
86	Gupta K, Banerjee A and Saggar K, Ahluwalia A and Saggar K: A prospective study of magnetic resonance imaging patterns of central nervous system infections in pediatric age group and young adults and their clinico-biochemical correlation. J Pediatr Neurosci. 11:46–51. 2016. View Article : Google Scholar : PubMed/NCBI
87	Acosta JH, Rantes CI, Arbelaez A, Restrepo F and Castillo M: Noncongenital central nervous system infections in children: Radiology review. Top Magn Reson Imaging. 23:153–164. 2014. View Article : Google Scholar : PubMed/NCBI
88	Papatheodoropoulou AE: Viral infections of central nervous system in children. Int J Mol Med. 40:S402017.
89	Koyuncu OO, Hogue IB and Enquist LW: Virus infections in the nervous system. Cell Host Microbe. 13:379–393. 2013. View Article : Google Scholar : PubMed/NCBI
90	Teoh HL, Mohammad SS, Britton PN, Kandula T, Lorentzos MS, Booy R, Jones CA, Rawlinson W, Ramachandran V, Rodriguez ML, et al: Clinical characteristics and functional motor outcomes of Enterovirus 71 neurological disease in children. JAMA Neurol. 73:300–307. 2016. View Article : Google Scholar : PubMed/NCBI
91	Erasmus M: Viral infections of Central Nervous System. CME. 29:190–193. 2011.
92	Venkatesan A, Tunkel AR, Bloch KC, Lauring AS, Sejvar J, Bitnun A, Stahl JP, Mailles A, Drebot M, Rupprecht CE, et al International Encephalitis Consortium: Case definitions, diagnostic algorithms, and priorities in encephalitis: Consensus statement of the international encephalitis consortium. Clin Infect Dis. 57:1114–1128. 2013. View Article : Google Scholar : PubMed/NCBI
93	Kneen R, Michael BD, Menson E, Mehta B, Easton A, Hemingway C, Klapper PE, Vincent A, Lim M, Carrol E, et al National Encephalitis Guidelines Development and Stakeholder Groups: Management of suspected viral encephalitis in children - Association of British Neurologists and British Paediatric Allergy, Immunology and Infection Group national guidelines. J Infect. 64:449–477. 2012. View Article : Google Scholar
94	Pillai SC, Hacohen Y, Tantsis E, Prelog K, Merheb V, Kesson A, Barnes E, Gill D, Webster R, Menezes M, et al: Infectious and autoantibody-associated encephalitis: Clinical features and long-term outcome. Pediatrics. 135:e974–e984. 2015. View Article : Google Scholar : PubMed/NCBI
95	Koutsaftiki C: RSV and rhinovirus infection in early childhood and their role in asthma. Int J Mol Med. 40:S402017.
96	Gidaris D, Urquhart D and Anthracopoulos MB: ‘They said it was bronchiolitis; is it going to turn into asthma doctor?’. Respirology. 19:1158–1164. 2014. View Article : Google Scholar : PubMed/NCBI
97	Kloepfer KM and Gern JE: Virus/allergen interactions and exacerbations of asthma. Immunol Allergy Clin North Am. 30:553–563. vii2010. View Article : Google Scholar : PubMed/NCBI
98	Chong Neto HJ, Rosario N, Solé D and Mallol J: Associated factors for recurrent wheezing in infancy. Allergy. 65:406–407. 2010. View Article : Google Scholar
99	Jackson DJ, Gangnon RE, Evans MD, Roberg KA, Anderson EL, Pappas TE, Printz MC, Lee WM, Shult PA, Reisdorf E, et al: Wheezing rhinovirus illnesses in early life predict asthma development in high-risk children. Am J Respir Crit Care Med. 178:667–672. 2008. View Article : Google Scholar : PubMed/NCBI
100	Kakumanu S: Virus-induced wheezing and asthma: An overview. UpToDate. 2017, https://www.uptodate.com/contents/virus-induced-wheezing-and-asthma-an-overview.
101	Gern JE: Viral respiratory infection and the link to asthma. Pediatr Infect Dis J. 27(10 Suppl): S97–S103. 2008. View Article : Google Scholar : PubMed/NCBI
102	Rantala AK, Jaakkola MS, Mäkikyrö EM, Hugg TT and Jaakkola JJ: Early respiratory infections and the development of asthma in the first 27 years of life. Am J Epidemiol. 182:615–623. 2015. View Article : Google Scholar : PubMed/NCBI
103	Gern JE: The ABCs of rhinoviruses, wheezing, and asthma. J Virol. 84:7418–7426. 2010. View Article : Google Scholar : PubMed/NCBI
104	Bartlett NW, McLean GR, Chang YS and Johnston SL: Genetics and epidemiology: Asthma and infection. Curr Opin Allergy Clin Immunol. 9:395–400. 2009. View Article : Google Scholar : PubMed/NCBI
105	Blanken MO, Rovers MM, Molenaar JM, Winkler-Seinstra PL, Meijer A, Kimpen JL and Bont L; Dutch RSV Neonatal Network: Respiratory syncytial virus and recurrent wheeze in healthy preterm infants. N Engl J Med. 368:1791–1799. 2013. View Article : Google Scholar : PubMed/NCBI
106	Stone CA Jr and Miller EK: Understanding the association of human rhinovirus with asthma. Clin Vaccine Immunol. 23:6–10. 2015. View Article : Google Scholar : PubMed/NCBI
107	Liston MA: The causes and contexts of perinatal mortality in ancient Greece: A case study from the ‘bone well’ of the Athenian Agora. Int J Mol Med. 40:S402017.
108	Liston MA, Rotroff SI and Snyder LM: The Agora bone well. Hesperia Supplements Am Sch Class Stud Athens. In press.
109	Liston MA and Rotroff SI: Babies in the well: Archaeological evidence for newborn disposal in Hellenistic Greece. The Oxford Handbook of Childhood and Education in the Classical World. Parkin T and Evans-Grubbs J: Oxford University Press; Oxford: 2013, https://doi.org/10.1093/oxfordhb/9780199781546.013.003.
110	Scheuer L and Black S: Developmental Juvenile Osteology. Elsevier Academic Press; London: 2000
111	Becker MJ: Etruscan infants: children’s cemeteries at Tarquinia, Italy, as indicators of an age of transition. (Re)thinking the Little Ancestor: New Perspectives on the Archaeology of Infancy and Childhood. Lally M and Moore A: Archaeopress; Oxford: 2011
112	Lewis ME: Endocranial lesions in non-adult skeletons: Understanding their aetiology. Int J Osteoarchaeol. 14:82–97. 2004. View Article : Google Scholar
113	Santos RP and Tristram D: A practical guide to the diagnosis, treatment, and prevention of neonatal infections. Pediatr Clin North Am. 62:491–508. 2015. View Article : Google Scholar : PubMed/NCBI
114	Mammas IN and Spandidos DA: Paediatric Virology: Focusing on medical education and training research. Int J Mol Med. 40:S252017.
115	Plutarch: Vioi Paralliloi; Solon - Poplikolas. 1. Kaktos Editions; 1992, In Greek.
116	European Academy of Paediatrics: Continuous Medical Education (CME). 2017, http://eapaediatrics.eu/working-group/continuous-medical-education-cme/.
117	Royal College of Paediatrics and Child Health: Continuing Professional Development (CPD). 2017, https://www.rcpch.ac.uk/training-examinations-professional-development/continuing-professional-development-cpd/continuing-pr.
118	Confederation of European Specialists in Paediatrics Working Group on Continuing Medical Education: Charter on continuing medical education in paediatrics in the European Union. Eur J Pediatr. 157:192–193. 1998. View Article : Google Scholar
119	Djuricich AM, Turco MG and Sachdeva AK: Changing continuing medical education. JAMA. 314:1072–1073. 2015. View Article : Google Scholar : PubMed/NCBI
120	Holl RA and Alden ER: Continuing medical education in Europe. Pediatr Rev. 23:335–336. 2002. View Article : Google Scholar : PubMed/NCBI
121	Costa A, Van Hemelryck F, Aparicio A, Gatzemeier W, Leer JW, Maillet B and Hossfeld DK: Continuing medical education in Europe: Towards a harmonised system. Eur J Cancer. 46:2340–2343. 2010. View Article : Google Scholar : PubMed/NCBI
122	James E: Clinical education in paediatrics. J Child Health Care. 2:178–181. 1998. View Article : Google Scholar
123	Mammas IN, Theodoridou M, Kramvis A, Thiagarajan P, Gardner S, Papaioannou G, Melidou A, Koutsaki M, Kostagianni G, Achtsidis V, et al: Paediatric Virology: A rapidly increasing educational challenge. Exp Ther Med. 13:364–377. 2017. View Article : Google Scholar : PubMed/NCBI