Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro Corrigendum in /10.3892/ijmm.2020.4743 Corrigendum in /10.3892/ijmm.2022.5146

Jiang,Xianfang; Zhong,Yanping; Zheng,Li; Zhao,Jinmin

doi:10.3892/ijmm.2018.3431

Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro

Corrigendum in: /10.3892/ijmm.2020.4743 Corrigendum in: /10.3892/ijmm.2022.5146

Authors:
- Xianfang Jiang
- Yanping Zhong
- Li Zheng
- Jinmin Zhao
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Affiliations: The College of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, The Medical and Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: January 26, 2018 https://doi.org/10.3892/ijmm.2018.3431
Pages: 2150-2158
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Autologous chondrocyte implantation (ACI) has emerged as a novel approach to cartilage repair through the use of harvested chondrocytes. However, the expansion of the chondrocytes from the donor tissue in vitro is restricted by the limited cell numbers and the dedifferentiation of the chondrocytes. The present study investigated the effect of collagen-based films, including collagen, hydroxyapatite (HA)/collagen (HC) and in situ synthesis of nano‑HC (nHC), on monolayer cultures of chondrocytes. As a substrate for the chondrocytes monolayer culture in vitro, nHC was able to restrain the dedifferentiation of chondrocytes and facilitate cell expansion, which was detected by methyl thiazolyl tetrazolium assay, scanning electron microscopy, calcein‑acetoxymethyl/propidium iodide staining, hematoxylin and eosin staining, Safranin O staining, immunohistochemical staining and reverse transcription‑quantitative polymerase chain reaction. Furthermore, the nHC films significantly facilitated cell growth and enhanced the expression of cartilage‑specific extracellular matrix (ECM) components, including aggrecan and type II collagen. In addition, nHC films markedly downregulated the expression of collagen type I, an indicator of dedifferentiation. The results indicated that nHC, a collagen‑based substrate optimized by nanoparticles, was able to better support cell growth and preserve cell phenotype compared with collagen alone or HC. The nHC film, which favors cell growth and prevents the dedifferentiation of chondrocytes, may therefore serve as a useful cartilage‑like ECM for chondrocytes. In conclusion, nHC film is a promising substrate for the culture of chondrocytes in cell-based therapy.

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1	Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O and Peterson L: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med. 331:889–895. 1994. View Article : Google Scholar : PubMed/NCBI
2	Chung C and Burdick JA: Engineering cartilage tissue. Adv Drug Deliv Rev. 60:243–262. 2008. View Article : Google Scholar
3	Lee JI, Sato M, Kim HW and Mochida J: Transplantatation of scaffold-free spheroids composed of synovium-derived cells and chondrocytes for the treatment of cartilage defects of the knee. Eur Cell Mater. 22:275–290. 2011. View Article : Google Scholar : PubMed/NCBI
4	Vinatier C, Gauthier O, Fatimi A, Merceron C, Masson M, Moreau A, Moreau F, Fellah B, Weiss P and Guicheux J: An injectable cellulose-based hydrogel for the transfer of autologous nasal chondrocytes in articular cartilage defects. Biotechnol Bioeng. 102:1259–1267. 2009. View Article : Google Scholar
5	Knudson W and Loeser RF: CD44 and integrin matrix receptors participate in cartilage homeostasis. Cell Mol Life Sci. 59:36–44. 2002. View Article : Google Scholar : PubMed/NCBI
6	Weber GF, Bjerke MA and DeSimone DW: Integrins and cadherins join forces to form adhesive networks. J Cell Sci. 124:1183–1193. 2011. View Article : Google Scholar : PubMed/NCBI
7	Chen X and Gumbiner BM: Crosstalk between different adhesion molecules. Curr Opin Cell Biol. 18:572–578. 2006. View Article : Google Scholar : PubMed/NCBI
8	Risbud MV and Sittinger M: Tissue engineering: advances in in vitro cartilage generation. Trends Biotechnol. 20:351–356. 2002. View Article : Google Scholar : PubMed/NCBI
9	Levorson EJ, Hu O, Mountziaris PM, Kasper FK and Mikos AG: Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, part 2: construct devitalization and determination of chondroinductive capacity. Tissue Eng Part C Methods. 20:358–372. 2014. View Article : Google Scholar :
10	Callahan LA, Ganios AM, McBurney DL, Dilisio MF, Weiner SD, Horton WE Jr and Becker ML: ECM production of primary human and bovine chondrocytes in hybrid PEG hydrogels containing type I collagen and hyaluronic acid. Biomacromolecules. 13:1625–1631. 2012. View Article : Google Scholar : PubMed/NCBI
11	Lin Z, Willers C, Xu J and Zheng MH: The chondrocyte: biology and clinical application. Tissue Eng. 12:1971–1984. 2006. View Article : Google Scholar : PubMed/NCBI
12	Caron MM, Emans PJ, Coolsen MM, Voss L, Surtel DA, Cremers A, van Rhijn LW and Welting TJ: Redifferentiation of dedifferentiated human articular chondrocytes: comparison of 2D and 3D cultures. Osteoarthritis Cartilage. 20:1170–1178. 2012. View Article : Google Scholar : PubMed/NCBI
13	Tekari A, Luginbuehl R, Hofstetter W and Egli RJ: Chondrocytes expressing intracellular collagen type II enter the cell cycle and co-express collagen type I in monolayer culture. J Orthop Res. 32:1503–1511. 2014. View Article : Google Scholar : PubMed/NCBI
14	Mhanna R, Öztürk E, Schlink P and Zenobi-Wong M: Probing the microenvironmental conditions for induction of superficial zone protein expression. Osteoarthritis Cartilage. 21:1924–1932. 2013. View Article : Google Scholar : PubMed/NCBI
15	Luo T and Kiick KL: Collagen-like peptides and peptide-polymer conjugates in the design of assembled materials. Eur Polym J. 49:2998–3009. 2013. View Article : Google Scholar : PubMed/NCBI
16	Guo Y, Yuan T, Xiao Z, Tang P, Xiao Y, Fan Y and Zhang X: Hydrogels of collagen/chondroitin sulfate/hyaluronan interpenetrating polymer network for cartilage tissue engineering. J Mater Sci Mater Med. 23:2267–2279. 2012. View Article : Google Scholar : PubMed/NCBI
17	Lee CH, Singla A and Lee Y: Biomedical applications of collagen. Int J Pharm. 221:1–22. 2001. View Article : Google Scholar : PubMed/NCBI
18	Goo HC, Hwang YS, Choi YR, Cho HN and Suh H: Development of collagenase-resistant collagen and its interaction with adult human dermal fibroblasts. Biomaterials. 24:5099–5113. 2003. View Article : Google Scholar : PubMed/NCBI
19	Kino-Oka M, Yashiki S, Ota Y, Mushiaki Y, Sugawara K, Yamamoto T, Takezawa T and Taya M: Subculture of chondrocytes on a collagen type I-coated substrate with suppressed cellular dedifferentiation. Tissue Eng. 11:597–608. 2005. View Article : Google Scholar : PubMed/NCBI
20	Gillogly SD and Wheeler KS: Autologous chondrocyte implantation with collagen membrane. Sports Med Arthrosc Rev. 23:118–124. 2015. View Article : Google Scholar : PubMed/NCBI
21	Hindle P, Hall AC and Biant LC: Viability of chondrocytes seeded onto a collagen I/III membrane for matrix-induced autologous chondrocyte implantation. J Orthop Res. 32:1495–1502. 2014. View Article : Google Scholar : PubMed/NCBI
22	Zheng L, Fan HS, Sun J, Chen XN, Wang G, Zhang L, Fan YJ and Zhang XD: Chondrogenic differentiation of mesenchymal stem cells induced by collagen-based hydrogel: an in vivo study. J Biomed Mater Res A. 93:783–792. 2010.
23	Li YY, Cheng HW, Cheung KM, Chan D and Chan BP: Mesenchymal stem cell-collagen microspheres for articular cartilage repair: cell density and differentiation status. Acta Biomater. 10:1919–1929. 2014. View Article : Google Scholar : PubMed/NCBI
24	Li W, Guo R, Lan Y and Zhang Y, Xue W and Zhang Y: Preparation and properties of cellulose nanocrystals reinforced collagen composite films. J Biomed Mater Res A. 102:1131–1139. 2014. View Article : Google Scholar
25	Zheng L, Jiang X, Chen X, Fan H and Zhang X: Evaluation of novel in situ synthesized nano-hydroxyapatite/collagen/alginate hydrogels for osteochondral tissue engineering. Biomed Mater. 9:0650042014. View Article : Google Scholar : PubMed/NCBI
26	Laydi F, Rahouadj R, Cauchois G, Stoltz JF and de Isla N: Hydroxyapatite incorporated into collagen gels for mesenchymal stem cell culture. Biomed Mater Eng. 23:311–315. 2013.PubMed/NCBI
27	Wang X, Grogan SP, Rieser F, Winkelmann V, Maquet V, Berge ML and Mainil-Varlet P: Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study. Biomaterials. 25:3681–3688. 2004. View Article : Google Scholar : PubMed/NCBI
28	Liang JZ: Reinforcement and quantitative description of inorganic particulate-filled polymer composites. Composites Part B Engineering. 51:224–232. 2013. View Article : Google Scholar
29	Fabbri P, Bondioli F, Messori M, Bartoli C, Dinucci D and Chiellini F: Porous scaffolds of polycaprolactone reinforced with in situ generated hydroxyapatite for bone tissue engineering. J Mater Sci Mater Med. 21:343–351. 2010. View Article : Google Scholar
30	Livak and Schmittgen: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar
31	Yashiki S, Umegaki R, Kino-Oka M and Taya M: Evaluation of attachment and growth of anchorage-dependent cells on culture surfaces with type I collagen coating. J Biosci Bioeng. 92:385–388. 2001. View Article : Google Scholar
32	Nixon AJ, Rickey E, Butler TJ, Scimeca MS, Moran N and Matthews GL: A chondrocyte infiltrated collagen type I/III membrane (MACI^® implant) improves cartilage healing in the equine patellofemoral joint model. Osteoarthritis Cartilage. 23:648–660. 2015. View Article : Google Scholar : PubMed/NCBI
33	Smeriglio P, Dhulipala L, Lai JH, Goodman SB, Dragoo JL, Smith RL, Maloney WJ, Yang F and Bhutani N: Collagen VI enhances cartilage tissue generation by stimulating chondrocyte proliferation. Tissue Eng Part A. 21:840–849. 2015. View Article : Google Scholar
34	Jia L, Duan Z, Fan D, Mi Y, Hui J and Chang L: Human-like collagen/nano-hydroxyapatite scaffolds for the culture of chondrocytes. Mater Sci Eng C. 33:727–734. 2013. View Article : Google Scholar
35	Hunter KT and Ma T: In vitro evaluation of hydroxyapatite-chitosan-gelatin composite membrane in guided tissue regeneration. J Biomed Mater Res A. 101:1016–1025. 2013. View Article : Google Scholar
36	Chen J, Yu Q, Zhang G, Yang S, Wu J and Zhang Q: Preparation and biocompatibility of nanohybrid scaffolds by in situ homogeneous formation of nano hydroxyapatite from biopolymer polyelectrolyte complex for bone repair applications. Colloids Surf B Biointerfaces. 93:100–107. 2012. View Article : Google Scholar : PubMed/NCBI
37	Chen J, Zhang G, Yang S, Li J, Jia H, Fang Z and Zhang Q: Effects of in situ and physical mixing on mechanical and bioactive behaviors of nano hydroxyapatite-chitosan scaffolds. J Biomater Sci Polym Ed. 22:2097–2106. 2011. View Article : Google Scholar
38	Zhang CY, Lu H, Zhuang Z, Wang XP and Fang QF: Nano-hydroxyapatite/poly(L-lactic acid) composite synthesized by a modified in situ precipitation: preparation and properties. J Mater Sci Mater Med. 21:3077–3083. 2010. View Article : Google Scholar : PubMed/NCBI
39	Chen J, Nan K, Yin S, Wang Y, Wu T and Zhang Q: Characterization and biocompatibility of nanohybrid scaffold prepared via in situ crystallization of hydroxyapatite in chitosan matrix. Colloids Surf B Biointerfaces. 81:640–647. 2010. View Article : Google Scholar : PubMed/NCBI
40	Zhu W, Chen K, Lu W, Sun Q, Peng L, Fen W, Li H, Ou Y, Liu H, Wang D, et al: In vitro study of nano-HA/PLLA composite scaffold for rabbit BMSC differentiation under TGF-β1 induction. In Vitro Cell Dev Biol Anim. 50:214–220. 2014. View Article : Google Scholar