MUTATIONS IN AN ALTERNATIVELY SPLICED EXON OF H-RAS ARE NOT ASSOCIATED WITH LOSS OF HETEROZYGOSITY ON CHROMOSOME 11P15.5 IN OVARIAN TUMORIGENESIS
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- Published online on: July 1, 1992 https://doi.org/10.3892/ijo.1.2.215
- Pages: 215-219
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Abstract
There is evidence that an alternatively spliced exon of the H-ras gene, called idx is associated with down-regulation of H-ras activity. We tested the hypothesis that mutations in this exon play an important role in the development of ovarian carcinomas because loss of heterozygosity at the H-ras locus is frequently observed in these tumors. The idx sequence of 26 different ovarian carcinomas was amplified by PCR and the products were analyzed for possible mutations by single-stranded conformation polymorphism and DNA sequencing. The results showed no idx mutations, even in tumors with a demonstrable loss of one H-ras allele.