Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas

  • Authors:
    • Thilo Papp
    • Holger Schipper
    • Heidi Pemsel
    • Ralf Bastrop
    • Klaus-Michael Muller
    • Thorsten Wiethege
    • Dieter G. Weiss
    • Elke Dopp
    • Dietmar Schiffmann
    • Qamar Rahman
  • View Affiliations

  • Published online on: February 1, 2001     https://doi.org/10.3892/ijo.18.2.425
  • Pages: 425-433
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Abstract

Nineteen specimens from primary human malignant mesotheliomas obtained from 19 patients were screened for activating point mutations in the oncogenes N-ras and CDK4 by combined RFLP-PCR/SSCP analysis. In addition, all tumours were screened for deletions and point mutations in the tumour suppressor genes p53, p16INK4a (CDKN2A) and p14ARF (exon-1β) by combined multiplex-PCR/SSCP analysis. No mutations were found in N-ras, p53 and CDK4. Three tumours displayed homozygous deletion (co-deletion of exons 1, 2 and 3) of p16INK4a. One of them displayed additional homozygous deletion of p14ARF (exon-1β). Two silent point mutations and 2 polymorphisms were found in p16INK4a in 3 tumours. Our preliminary data indicate that disarrangement of the Rb1 pathway may be involved in mesothelioma formation.

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February 2001
Volume 18 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Papp T, Schipper H, Pemsel H, Bastrop R, Muller K, Wiethege T, Weiss DG, Dopp E, Schiffmann D, Rahman Q, Rahman Q, et al: Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas. Int J Oncol 18: 425-433, 2001.
APA
Papp, T., Schipper, H., Pemsel, H., Bastrop, R., Muller, K., Wiethege, T. ... Rahman, Q. (2001). Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas. International Journal of Oncology, 18, 425-433. https://doi.org/10.3892/ijo.18.2.425
MLA
Papp, T., Schipper, H., Pemsel, H., Bastrop, R., Muller, K., Wiethege, T., Weiss, D. G., Dopp, E., Schiffmann, D., Rahman, Q."Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas". International Journal of Oncology 18.2 (2001): 425-433.
Chicago
Papp, T., Schipper, H., Pemsel, H., Bastrop, R., Muller, K., Wiethege, T., Weiss, D. G., Dopp, E., Schiffmann, D., Rahman, Q."Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas". International Journal of Oncology 18, no. 2 (2001): 425-433. https://doi.org/10.3892/ijo.18.2.425