Induction of apoptosis by a ribosome-inactivating protein from Agrostemma githago is associated with down-regulation of anti-apoptotic bcl-2 protein expression

  • Authors:
    • Lawrence C.M. Chiu
    • Vincent E.C. Ooi
    • S. S.M. Sun
  • View Affiliations

  • Published online on: July 1, 2001     https://doi.org/10.3892/ijo.19.1.137
  • Pages: 137-141
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Abstract

Agrostin is a type 1 ribosome-inactivating protein (RIP) isolated from the seeds of Agrostemma githago. 3[H]-thymidine incorporation in human leukemic HL-60 cells was significantly reduced after incubation with agrostin showing that the RIP was inhibitory on the cell proliferation. Results from flow cytometric studies illustrated that apoptosis was induced by agrostin, which was associated with activation of endonucleases and DNA fragmentation of the leukemic cells. Anti-apoptotic bcl-2 protein level was also reduced by agrostin showing that the associated apoptotic pathway might involve down-regulation of the intracellular bcl-2 protein level.

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July 2001
Volume 19 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chiu LC, Ooi VE and Sun SS: Induction of apoptosis by a ribosome-inactivating protein from Agrostemma githago is associated with down-regulation of anti-apoptotic bcl-2 protein expression. Int J Oncol 19: 137-141, 2001.
APA
Chiu, L.C., Ooi, V.E., & Sun, S.S. (2001). Induction of apoptosis by a ribosome-inactivating protein from Agrostemma githago is associated with down-regulation of anti-apoptotic bcl-2 protein expression. International Journal of Oncology, 19, 137-141. https://doi.org/10.3892/ijo.19.1.137
MLA
Chiu, L. C., Ooi, V. E., Sun, S. S."Induction of apoptosis by a ribosome-inactivating protein from Agrostemma githago is associated with down-regulation of anti-apoptotic bcl-2 protein expression". International Journal of Oncology 19.1 (2001): 137-141.
Chicago
Chiu, L. C., Ooi, V. E., Sun, S. S."Induction of apoptosis by a ribosome-inactivating protein from Agrostemma githago is associated with down-regulation of anti-apoptotic bcl-2 protein expression". International Journal of Oncology 19, no. 1 (2001): 137-141. https://doi.org/10.3892/ijo.19.1.137