Identification of signature genes by microarray for acute myeloid leukemia without maturation and acute promyelocytic leukemia with t(15;17)(q22;q12)(PML/RARα)

  • Authors:
    • Jun Morikawa
    • Huai Li
    • Seungchan Kim
    • Kazuhiro Nishi
    • Satoshi Ueno
    • Edward Suh
    • Edward Dougherty
    • Ilya Shmulevich
    • Hiroshi Shiku
    • Wei Zhang
    • Tohru Kobayashi
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/ijo.23.3.617
  • Pages: 617-625
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Abstract

Acute myeloid leukemia (AML) has distinct subgroups characterized by different maturation and specific chromosomal translocation. In order to gain insight into the gene expression activities in AML, we carried out a gene expression profiling study with 21 AML samples using cDNA microarrays, focusing on acute promyelocytic leukemia with specific translocation t(15;17)(q22;q12) [French-American-British or FAB-M3 with t(15;17)] and AML without maturation (FAB-M1) characterized by morphologically and phenotypically immature AML blasts and no recurrent chromosomal abnormalities. Using a multivariate σ-classifier algorithm, we identified 33 strong feature genes that distinguish FAB-M3 with t(15;17) from other AML samples, and 24 strong feature genes that classify FAB-M1. A direct comparison between FAB-M3 with t(15;17) and FAB-M1 led to selection of 13 strong feature genes. Those genes include some known to be related to leukemogenesis and cell differentiation. RIN1, a gene in the ras pathway, was up-regulated in FAB-M3 with t(15;17). Growth factor-binding protein 2 gene was down-regulated in FAB-M1. Huntingtin gene was up-regulated in FAB-M1. Others include syndecan 4, interleukin-2 receptor β, folate receptor β, low affinity immunoglobulin γ, Fc receptor IIC precursor, insulin-like growth factor binding protein 2, and myeloperoxidase, which are involved in cell differentiation. Overexpression of myeloperoxidase in FAB-M3 cells with t(15;17) compared to FAB-M1 cells is consistent with the conventional cytochemical staining pattern. Thus, the study revealed that a morphologically-defined FAB-M1 subtype has a distinct gene expression signature that contributes to its cell differentiation and proliferation as well as FAB-M3 with a recurrent cytogenetic abnormality t(15;17)(q22;q12).

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September 2003
Volume 23 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Morikawa J, Li H, Kim S, Nishi K, Ueno S, Suh E, Dougherty E, Shmulevich I, Shiku H, Zhang W, Zhang W, et al: Identification of signature genes by microarray for acute myeloid leukemia without maturation and acute promyelocytic leukemia with t(15;17)(q22;q12)(PML/RARα). Int J Oncol 23: 617-625, 2003.
APA
Morikawa, J., Li, H., Kim, S., Nishi, K., Ueno, S., Suh, E. ... Kobayashi, T. (2003). Identification of signature genes by microarray for acute myeloid leukemia without maturation and acute promyelocytic leukemia with t(15;17)(q22;q12)(PML/RARα). International Journal of Oncology, 23, 617-625. https://doi.org/10.3892/ijo.23.3.617
MLA
Morikawa, J., Li, H., Kim, S., Nishi, K., Ueno, S., Suh, E., Dougherty, E., Shmulevich, I., Shiku, H., Zhang, W., Kobayashi, T."Identification of signature genes by microarray for acute myeloid leukemia without maturation and acute promyelocytic leukemia with t(15;17)(q22;q12)(PML/RARα)". International Journal of Oncology 23.3 (2003): 617-625.
Chicago
Morikawa, J., Li, H., Kim, S., Nishi, K., Ueno, S., Suh, E., Dougherty, E., Shmulevich, I., Shiku, H., Zhang, W., Kobayashi, T."Identification of signature genes by microarray for acute myeloid leukemia without maturation and acute promyelocytic leukemia with t(15;17)(q22;q12)(PML/RARα)". International Journal of Oncology 23, no. 3 (2003): 617-625. https://doi.org/10.3892/ijo.23.3.617