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Article

MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer

  • Authors:
    • Berlinda Verdoodt
    • Matthias Neid
    • Markus Vogt
    • Viktoria Kuhn
    • Sven-Thorsten Liffers
    • Rein-Jüri Palisaar
    • Joachim Noldus
    • Andrea Tannapfel
    • Alireza Mirmohammadsadegh
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, Ruhr-University Bochum, D-44789 Bochum, Germany, Department of Urology, Marienhospital Herne, Ruhr-University Bochum, D-44627 Herne, Germany
  • Pages: 307-314
    |
    Published online on: April 23, 2013
       https://doi.org/10.3892/ijo.2013.1915
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Abstract

Decreased expression of the microRNA miR-205 has been observed in multiple tumour types due to its role in the epithelial to mesenchymal transition, which promotes metastasis. We determined the expression of miR-205 in 111 archival samples of prostate carcinoma and found it to be strongly reduced in most samples, with a median expression level of 16% in comparison to benign tissue from the same patient. Lower miR-205 expression correlated significantly with tumour size and miR-205 levels decreased with increasing Gleason score from 7a=3+4 to 8=4+4. In addition, we describe the anti-apoptotic protein BCL2 as a target of miR-205, relevant for prostate cancer due to its role in prognosis of primary tumours and in the appearance of androgen independence. The repression of BCL2 by miR-205 was confirmed using reporter assays and western blotting. BCL2 mRNA expression in the same collective of prostate cancer tissue samples was associated with higher Gleason score and extracapsular extension of the tumour (pT3). Consistent with its anti-apoptotic target BCL2, miR-205 promoted apoptosis in prostate cancer cells in response to DNA damage by cisplatin and doxorubicin in the prostate cancer cell lines PC3 and LnCap. MiR-205 also inhibited proliferation in these cell lines.
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Copy and paste a formatted citation
Spandidos Publications style
Verdoodt B, Neid M, Vogt M, Kuhn V, Liffers S, Palisaar R, Noldus J, Tannapfel A and Mirmohammadsadegh A: MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer. Int J Oncol 43: 307-314, 2013.
APA
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R. ... Mirmohammadsadegh, A. (2013). MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer. International Journal of Oncology, 43, 307-314. https://doi.org/10.3892/ijo.2013.1915
MLA
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R., Noldus, J., Tannapfel, A., Mirmohammadsadegh, A."MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer". International Journal of Oncology 43.1 (2013): 307-314.
Chicago
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R., Noldus, J., Tannapfel, A., Mirmohammadsadegh, A."MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer". International Journal of Oncology 43, no. 1 (2013): 307-314. https://doi.org/10.3892/ijo.2013.1915
Copy and paste a formatted citation
x
Spandidos Publications style
Verdoodt B, Neid M, Vogt M, Kuhn V, Liffers S, Palisaar R, Noldus J, Tannapfel A and Mirmohammadsadegh A: MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer. Int J Oncol 43: 307-314, 2013.
APA
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R. ... Mirmohammadsadegh, A. (2013). MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer. International Journal of Oncology, 43, 307-314. https://doi.org/10.3892/ijo.2013.1915
MLA
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R., Noldus, J., Tannapfel, A., Mirmohammadsadegh, A."MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer". International Journal of Oncology 43.1 (2013): 307-314.
Chicago
Verdoodt, B., Neid, M., Vogt, M., Kuhn, V., Liffers, S., Palisaar, R., Noldus, J., Tannapfel, A., Mirmohammadsadegh, A."MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer". International Journal of Oncology 43, no. 1 (2013): 307-314. https://doi.org/10.3892/ijo.2013.1915
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