Chromothripsis and progression‑free survival in metastatic colorectal cancer
- Elina Skuja
- Dagnija Kalniete
- Miki Nakazawa‑Miklasevica
- Zanda Daneberga
- Arnis Abolins
- Gunta Purkalne
- Edvins Miklasevics
- Published online on: January 2, 2017 https://doi.org/10.3892/mco.2017.1123
Copyright: © Skuja et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Mortality from colorectal cancer (CRC) has decreased over the past 30 years; however, there is a significant heterogeneity in survival rates that may be mainly explained by variations in patient and tumor characteristics, host response factors and applied treatment modalities. At the time of diagnosis, ~25% of the patients have already developed metastases and ~50% will develop metastases following treatment of the primary CRC (1).
During cancer development, the cells acquire multiple point mutations and chromosomal rearrangements. Chromosomal abnormalities that are found in primary CRC cells are gains of 8q, 11q, 13q and 20q and losses of 1p, 4, 8p, 17p, 18q and 22q chromosomal regions (2–5).
Van den Broek et al reported a clinical relevance of chromosomal breaks in metastatic CRC (mCRC) in 2015; they identified 1605 genomic breakpoint locations and 748 breakpoint genes with recurrence in multiple CRC samples. None of the individual breakpoint genes was significantly associated with overall survival (OS), but four CRC subtypes were revealed based on recurrent breakpoint genes and mutation status of commonly affected CRC genes [adenomatous polyposis coli, tumor protein p53, Kirsten rat sarcoma viral oncogene homolog (KRAS), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α, B-Raf proto-oncogene, serine/threonine kinase, neuroblastoma RAS viral oncogene homolog, mothers against decapentaplegic homolog 4 and F-box/WD repeat-containing protein 7] (6).
Chromothripsis, a massive chromosome fragmentation occurring in one catastrophic event, is observed in 2–3% of cancers (7). Such genomic rearrangements may drive the development of cancer through several mechanisms, including deletion of tumor suppressor genes and increased copy number of oncogenes. The prevalence of chromothripsis and its effect on prognosis and metastasis is unclear. The incidence of chromothripsis ranges from 1.3% in multiple myeloma (8) to 33% in osteosarcoma (7). Furthermore, chromothripsis has been associated with poor patient survival in the aggressive triple-negative subtype of breast cancer, multiple myeloma and pediatric medulloblastoma (8–10), indicating its potential relevance as a prognostic marker, and suggesting chromothripsis to be a characteristic of certain particularly aggressive types of cancer.
The incidence of chromothripsis and its effect on survival in CRC is unclear, but it may be a prevalent genomic rearrangement in this type of cancer (11).
Patients and methods
A total of 19 mCRC patients who received chemotherapy at the Clinic of Oncology of Pauls Stradins Clinical University Hospital (Riga, Latvia) between August, 2011 and October, 2012 were selected. The study was performed with approval from the Ethics Committee of the Riga Stradins University and written informed consent was obtained from all the patients who were investigated. Tissue samples were acquired as part of a series of routine diagnostic and pathological analyses at the hospital. The patients were followed up for 3–48 months.
The clinical and biological characteristics of the patients are summarized in Table I. Of the 19 patients, 15 had primary metastatic cancer (stage IV), whereas the 4 remaining patients developed metastases after treatment of the primary cancer. A total of 10 patients developed metastases only to the liver; 16 patients underwent primary tumor surgery, whereas in 3 patients biopsy alone was performed. In 7 patients, the carcinoembryonic antigen (CEA) level was ≤5.5 ng/ml prior to chemotherapy.
A total of 18 patients received FOLFOX first-line chemotherapy (1 patient declined chemotherapy). After disease progression, 13 patients received irinotecan-containing second-line chemotherapy, 1 patient was rechallenged with FOLFOX, 1 patient received oral fluoropyrimidine therapy with Ftorafur (tegafur) and 1 patient received best supportive care. Only 5 patients (26.3%) received third-line therapy [irinotecan, oxaliplatin or 5-fluorouracil (5FU)]. One patient underwent hepatic surgery for CRC metastases after discontinuation of second-line chemotherapy, 2 patients received salvage transcatheter arterial chemoembolization of CRC liver metastases by irinotecan-eluting microspheres, and 1 patient received palliative radiotherapy for local rectal cancer. Data on clinical follow-up were obtained until August, 2016.
DNA was extracted from formalin-fixed paraffin-embedded (FFPE) samples with QIAamp DNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Quality was evaluated using the Illumina FFPE QC kit (Illumina, San Diego, CA, USA) by reverse transcription-polymerase chain reaction. DNA was restored with the Illumina DNA restoration kit (Illumina). Microarray analysis was performed using the Infinium HumanOmniExpress-12 v1.0 FFPE BeadChip kit (Illumina). BeadChip was scaned on HiScan (Illumina). Analysis was performed by GenomeStudio software (Illumina) and R version 3.1.2. (https://www.r-project.org/). Copy number variation and breakpoints on the chromosomes were analyzed using the DNA copy package (http://bioconductor.org/packages/release/bioc/html/DNAcopy.html).
OS and progression-free survival (PFS) rates were estimated using the Kaplan-Meier method. The log-rank test was used to calculate any significant difference between the subgroups by univariate analysis. Significance levels were set at P<0.05. All statistical analyses were performed using MedCalc software, version 16.4.8 (MedCalc Software, Ostend, Belgium).
Breakpoint count per chromosome
The aim of this study was to evaluate the total number of chromosomal breaks and association of chromothripsis (>100 breakpoints detected in one chromosome) with PFS in mCRC. The total number of breakpoints per genome in cancer tissue [breakpoint instability index (BPI)] was 368–4,009. The highest breakpoint count was seen in chromosome 1 (27–365 breakpoints), followed by chromosome 2 (25–315) (Table II), indicating the crucial role of these chromosomes in CRC development. The lowest density of breaks occurred in chromosome 21 (7–99 breakpoints; Fig. 1). Of note, a similar pattern of chromosomal rearrangements was observed in several patients for chromosomes 1, 3 and 8 (Fig. 2).
Multiple chromosome 1 fragmentation (chromothripsis). There were multiple deletions in both arms of chromosomes 1 and 2 in patient 14. Chr, chromosome; CN, copy number.
Similar pattern of chromosomal rearrangements of chromosome 3 in four different patients (patients 3, 8, 9 and 14). Chr, chromosome; CN, copy number.
In 10 of the tumor samples (52.6%), multiple chromosomal fragmentations were found, found, referred to as chromothripsis, a recently reported catastrophic genetic event. The most commonly affected chromosomes were chromosomes 1, 2 and 6 (52.6% of the patients; Table II). The maximal count of chromosomes affected by chromothripsis was 20, which was observed in 1 patient.
Association of breakpoint number with clinicopathological characteristics
No association of BPI value and chromothripsis with cancer localization (rectal or colon cancer), CEA level, KRAS mutational status in primary cancer and stage [synchronous metastatic disease (stage IV) vs. metachronous metastatic disease] was observed.
Association of breakpoint number with survival
PFS and OS were measured for all the patients in study. Decreased PFS was observed in patients with left-sided metastatic cancer (sigmoid colon and rectal cancer), elevated CEA level prior to chemotherapy (Fig. 3A) and KRAS mutations.
(A) mPFS in elevated CEA: 8 months; mPFS in CEA ≤5.5 ng/ml: 14 months; HR=0.29. (B) mPFS for BPI ≥1,400: 14 months; mPFS for BPI <1,400: 8 months; HR=3.43. (C) mPFS in cases exhibiting chromothripsis (+): 14 months; mPFS in cases without chromothripsis (−): 8 months; HR=3.43. mPFS, median progression-free survival; CEA, carcinoembryonic antigen; BPI, breakpoint instability index; HR, hazard ratio.
A positive correlation between high BPI (chromothripsis) and increased PFS was observed (Fig. 3B and C). Due to the observed better PFS in different clinical subgroups by CEA, KRAS status and primary tumor location, the effect of BPI and chromothripsis on clinical findings was analyzed; however, the number of patients in each subgroup was insufficient to establish a statistically significant correlation. In patients with detected chromothripsis and CEA ≤5.5 ng/ml (n=4), a median PFS (mPFS) of 22.5 months was observed; in patients without chromothripsis and elevated CEA level (n=7), an mPFS of 8 months was observed, but these findings did not reach statistical significance. A statistically significant effect of any clinical or biological factors on OS was not observed.
The effect of chromosomal rearrangements and mutations on pathogenesis, prognosis and resistance to treatment are widely described in mCRC studies. The prognostic and predictive role of breakpoint instability index and chromothripsis remains unclear.
In the present study, a correlation between massive DNA fragmentation (chromothripsis) and PFS in mCRC was observed. As opposed to recent studies suggesting chromothripsis to be associated with worse prognosis, we found chromothripsis to be a positive predictive factor for first-line chemotherapy. It may be hypothesized that cancer cells exhibiting radical DNA rearrangements, such as chromothripsis, are more sensitive to nucleic acid-damaging therapy with 5FU and oxaliplatin.
Breakpoint instability index was previously reported in breast cancer study by Przybytkowski et al (10); they reported a BPI of 25–300 in breast cancer tissue, with the highest density of breaks in chromosome 17 and the lowest density in chromosome 4. The BPI appeared to be different in different breast cancer molecular subtypes, with the highest breakpoint count in aggressive triple-negative breast cancer. In comparison, in our study on CRC tissue, the highest breakpoint density was found in chromosomes 1 and 2 and the lowest in chromosome 21, but the BPI was significantly higher (368–4,009). In addition, 10 tumor samples (52.6%) exhibited a chromothripsis pattern on ≥3 chromosomes, which was higher compared with previous reports (7,8,10). The high BPI value and high prevalence of chromothripsis in our study may be attributed to the fact that all the patients had late-stage metastatic disease, which is consistent with the prevalence of chromothripsis reported in high-risk aggressive tumors.
In conclusion, the present study demonstrated that chromothripsis is associated with increased PFS, but not with OS in mCRC. Further studies with larger sample sizes are required to determine whether chromothripsis and BPI value may be used as a prognostic or predictive factor for oxaliplatin-containing first-line chemotherapy for mCRC.
The present study was partly supported by the National Research Program ‘Biomedicine for Public Health (BIOMEDICINE)’.
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