Spontaneous splenic rupture in an acute leukemia patient with splenic tuberculosis: A case report

  • Authors:
    • Yue Zhang
    • Juan Zhang
    • Tingting Chen
    • Hui Zeng
    • Bing Zhao
    • Yong Zhang
    • Xiaohuan Zhou
    • Wei Han
    • Yanping Hu
    • Fengge Liu
    • Zhijuan Shan
    • Weifeng Gao
    • Hebing Zhou
  • View Affiliations

  • Published online on: December 28, 2016     https://doi.org/10.3892/mco.2016.1120
  • Pages:209-213
0

Abstract

Spontaneous splenic rupture, also referred to as atraumatic splenic rupture, is a rare but life‑threatening emergency condition. Without timely diagnosis and treatment, the mortality rate of splenic rupture approaches 100%. The etiology of atraumatic splenic rupture varies; it is reportedly associated with neoplasms or splenic infection, but is rarely encountered in patients with both conditions. We herein report the case of a 58‑year‑old male patient with acute myeloid leukemia (AML) complicated by splenic tuberculosis (TB), who presented with spontaneous rupture of the spleen. Pathological examination of the resected spleen revealed multifocal granulomatosis with caseous necrosis. However, with timely diagnosis and surgical intervention, the patient recovered successfully and is currently on consolidation therapy. To the best of our knowledge, this is the first case of spontaneous splenic rupture in AML with splenic TB. The relevant literature on spontaneous splenic rupture was also reviewed and the potential etiology and treatment were discussed.

Introduction

Spontaneous splenic rupture, also referred to as atraumatic splenic rupture, is an uncommon serious complication of acute leukemia, with very few reported cases since Rokitansky first described spontaneous rupture of the spleen in a patient with leukemia in 1861 (17). Although the precise occurrence of spontaneous splenic rupture is not known, its actual morbidity may be higher due to the difficulty of diagnosis in the past. In the past, the mortality rate of splenic rupture was extremely high, due to the patient's poor general condition, complications and difficulty in diagnosis, with the major cause of death being hypotensive shock from the extensive blood loss, whereas infection and other complications also played a major role. However, with the increased availability of imaging modalities, the mortality rate has been reduced significantly over the past decades. In the present study, we report a case of spontaneous splenic rupture occurring in a patient with acute myeloid leukemia (AML).

Case report

A 58-year-old male patient was admitted to the Luhe Hospital (Beijing, China) in July, 2015, with a 2-month history of fever and cough, without symptoms of fatigue or night sweats. The patient had no history of TB or human immunodeficiency virus infection. The physical examination was unremarkable. The peripheral blood smear revealed pancytopenia [hemoglobin (HGB) level of 83 g/l, white blood cell (WBC) count of 2.92×109/l and platelet (PLT) count of 61×109/l], with 22% blasts. Certain blast cells included Auer rods. The bone marrow smear was characterized by hypercellularity and patches of myeloblasts with Auer rods (Fig. 1). These results were consistent with AML of the M2 subtype. Following the diagnosis of leukemia, leukemia genetic screening was performed. The gene screening was negative. The c-KIT exon 8, c-KIT exon 17, FMS-like tyrosine kinase 3 internal tandem duplication and nucleophosmin 1 were all wild-type, but Janus kinase (JAK) 2V617F or calreticulin (CALR) gene detection was not performed. Bone marrow conventional cytogenetic analysis on the G-banded metaphases revealed the following: 46,XY, del (20)(q11)(20). The results of fluorescence in situ hybridization were as follows: AML1/ETO:RUNX/RUNX1T1 0%.

The patient achieved complete remission after induction chemotherapy with a regimen comprising daunorubicin 60 mg/m2 on days 1–3 and cytarabine 200 mg/m2 on days 1–7; however, he developed severe myelosuppression, agranulocytosis, and was febrile for 3 weeks. The galactomannan assay was 56.10 pg/ml. Bronchoalveolar lavage revealed a few bronchial epithelia and multifocal neutrophilic infiltration. An abdominal and pelvic CT scan revealed no hepatosplenomegaly. The patient was empirically treated with broad-spectrum antibiotics (imipenem and cilastatin sodium for injection, vancomycin hydrochloride for injection, cefoperazone sodium and sulbactam sodium for injection) and antifungal agents [voriconazole for injection (DSM Pharmaceuticals, Inc., Greenville, NC, USA) and amphotericin B (North China Pharmaceutical Co., Ltd., Shijiazhuang, China)]. A repeat chest CT showed improvement of the infiltrates and his temperature normalized.

After the second course of chemotherapy, the peripheral blood smear revealed a WBC count of 4.84×109/l with a small number of immature WBCs, an HGB level of 106 g/l and a PLT count of 335×109/l. On repeat bone marrow biopsy, dry tap aspiration, focal granulopoietic progenitors and abnormal localization of immature precursors were observed. The red cell population consisted mainly of intermediate- and late-stage erythroblasts. The number of multinucleated megakaryocytes was increased (8–12/high-power field). The reticulin fibres were focally proliferated and were positive (++) on Gömöri trichrome staining. On immunocytochemical staining, there was a small number of CD34+ precursor cells, the leukemic blasts were myeloperoxidase (MPO)+ and fractional leukemic blasts were CD117+. The Ki-67 index was 30% (Fig. 2). Interestingly, a homozygous JAK2V617F mutation was detected by polymerase chain reaction (PCR), as well as a missense mutation in exon 9 of the CALR gene by bidirectional sequencing. The latter mutation has been classified as c.1142A>C (p.E381A). The results were consistent with AML with myelofibrosis (MF).

A third course of chemotherapy with mitoxantrone 4 mg/m2 on days 1–3 and cytarabine 150 mg/m2 on days 1–7 was subsequently administered. On day 3, the patient developed sudden-onset nausea, dizziness and severe abdominal pain, he quickly became hypotensive and the HGB level decreased to 8.3 g/dl within 1 h. The coagulation tests (prothrombin time, activated partial thromboplastin time, thrombin time and fibrinogen) were within the normal range. The patient received aggressive fluid resuscitation, vasopressors (dopamine; Shanghai Hefeng Pharmaceutical Co., Ltd., Shanghai, China), and was intubated for respiratory support. Chemotherapy was discontinued after a total dose of 8 mg/m2 of mitoxantrone and 300 mg/m2 of cytarabine. An abdominal ultrasound revealed a considerable amount of free fluid surrounding the liver and spleen, with a density consistent with that of blood. An emergency laparotomy confirmed splenic rupture and splenectomy was performed during the procedure. Approximately 4,000 ml of fresh blood were evacuated from the abdominal cavity. The patient was intubated and transferred to the intensive care unit. After 1 week, the patient was extubated and he eventually recovered fully.

Pathological examination of the resected ruptured spleen revealed increased red pulp, decreased white pulp and multifocal granulomatosis with caseous necrosis, which raised the suspicion of TB; however, the acid-fast staining was negative. Furthermore, immunohistochemical staining of the splenic tissue revealed the presence of some MPO-positive cells, but they were negative for CD34 and CD117. The Ki-67 was sparsely positive in a few splenic cells. The periodic acid-Schiff staining was negative (Fig. 3). JAK2V617F mutation in the splenic specimens was negative by the PCR method.

A purified protein derivative test was strongly positive. The erythrocyte sedimentation rate was 128 mm/h. The T-SPOT.TB test was negative. The patient was empirically treated for TB with a combination of isoniazid, rifampicin, ethambutol and pyrazinamide, despite the acid-fast staining being negative. On bone marrow biopsy 1 month later, there was no dry tap aspiration and complete remission was achieved. However, JAK2V617 and CALR mutations were still detected. The patient then received consolidation chemotherapy with standard-dose cytarabine (Pfizer Italia Srl, Rome, Italy), daunorubicin (Haizheng Pharmaceutical Co., Ltd., Taizhou, China) or mitoxantrone (Jiangsu Hengrui Pharmaceutical Co., Lianyungang, China). The last follow-up was in October, 2016, and the patient exhibited complete remission of the bone marrow.

Discussion

Spontaneous splenic rupture refers to rupture of the spleen without a history of blunt or penetrating trauma. Although rare, spontaneous splenic rupture may be associated with neoplastic (30.3%), infectious (27.3%), inflammatory, non-infectious (20.0%), drug- and treatment-related (9.2%) and mechanical (6.8%) disorders; it may also occur in the normal spleen (6.4%) (8). The major symptoms and signs of splenic rupture include abdominal pain, tenderness and guarding, hypotension, nausea, vomiting, dizziness and syncope (9). However, no characteristic clinical manifestation may be used to definitively diagnose splenic rupture without further investigation. The definitive treatment for spontaneous splenic rupture is emergent splenectomy. Without splenectomy, the mortality rate in such patients approaches 100% (10).

While atraumatic splenic rupture most commonly occurs in association with neoplastic diseases, its mechanism has not been fully elucidated. In 1964, Hynes et al (11) proposed three possible mechanisms underlying spontaneous splenic rupture, which remain widely accepted to date: Mechanical effect of distention secondary to leukemic infiltration of the spleen, particularly the capsule; splenic infarct with capsular hemorrhage and subsequent rupture; and blood coagulation defects. However, it appears that none of the abovementioned mechanisms was responsible for the spontaneous splenic rupture in our patient. First, his spleen was not infiltrated by leukemic cells on pathological examination, which was confirmed by negative immunohistochemical staining for CD117 and CD34, and the splenic tissue was negative for the JAK2V617F mutation. Moreover, there was no evidence of blood coagulopathy or splenic infarct. Therefore, the patient's splenic rupture cannot be explained by leukemia or intracapsular hemorrhage.

The splenic rupture in our patient likely involved other underlying mechanisms. First, the pathological examination of the ruptured spleen revealed findings considered to be typical for TB. Splenic TB is an extremely rare cause of splenic rupture, with only 3 cases of splenic abscess and rupture as a consequence of TB infection described in the literature to date (12,13). TB mostly locates primarily in the lungs, and splenic TB is a rare form of extrapulmonary localization. Immunocompromised patients, such as those with acquired immunodeficiency syndrome, have been reported to be at high risk for splenic TB (14), but the diagnosis may be challenging due to the non-specific clinical manifestations. The patient may suffer from fever, left upper quadrant abdominal pain, weight loss, diarrhea and, occasionally, ascites (15). However, in one-third of the cases, there were no abdominal symptoms, despite sonographic confirmation of a splenic lesion (16). Therefore, the diagnosis of splenic TB is difficult and is often missed or delayed. In patients with TB, splenic TB may present as multiple small hypoechoic lesions on abdominal sonography, or hypodense lesions on abdominal CT (17). Diagnosis is established by pathological examination of the fine-needle aspirate, splenic biopsy, or examination of the surgical specimen following splenectomy (18). However, invasive diagnostic procedures are difficult to perform in hematological patients due to the increased risk of bleeding and marked neutropenia following intensive chemotherapy. Therefore, management is largely dependent on clinical diagnosis when limited microbiological data are available (19).

On the other hand, the patient may be diagnosed with AML with primary MF (PMF). The bone marrow biopsy revealed dry tap aspiration, increased megakaryocyte number and focal hyperplasia of the fibrous tissue, and was positive for Gömöri trichrome staining, JAK2V617 and CALR mutations. The activating mutation JAK2V617F is frequently found in myeloproliferative neoplasms (MPNs), including polycythemia vera, essential thrombocytosis and PMF (20). MPNs share an increased risk of thrombotic and hemorrhagic complications. Thrombosis occurs in less typical sites, including cerebral sinus vessels and splanchnic vessels (hepatic, portal, mesenteric and splenic veins). Emerging risk factors may include leukocytosis and presence of the JAK2V617F mutation, or an increase in its allelic burden. Hemorrhagic complications are likely associated with acquired platelet defects, such as acquired von Willebrand syndrome (21). Whether thrombotic and hemorrhagic complications caused by MPNs are associated with splenic rupture remains to be further elucidated. Although splenectomy is an effective treatment for MPN-related symptoms, it is also associated with significant operative morbidity, increased mortality, and even a potential increased risk of blast phase transformation in MF (22,23). Most clinicians manage to avoid splenectomy in the majority of the patients.

In conclusion, it is hypothesized that the splenic rupture in our patient was caused by the subcapsular localization of tuberculous granulomatous tissue infiltrating the red pulp of the spleen, predisposing to tearing of the splenic capsule. The role of MPN in the splenic rupture may be another, although unlikely, possibility, since there was no evidence of coagulopathy. Regardless of the underlying mechanism, sudden onset of abdominal pain associated with symptomatic anemia or hypotension in a patient with leukemia warrants further investigation to exclude fatal splenic rupture.

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APA
Zhang, Y., Zhang, J., Chen, T., Zeng, H., Zhao, B., Zhang, Y. ... Zhou, H. (2017). Spontaneous splenic rupture in an acute leukemia patient with splenic tuberculosis: A case report. Molecular and Clinical Oncology, 6, 209-213. https://doi.org/10.3892/mco.2016.1120
MLA
Zhang, Y., Zhang, J., Chen, T., Zeng, H., Zhao, B., Zhang, Y., Zhou, X., Han, W., Hu, Y., Liu, F., Shan, Z., Gao, W., Zhou, H."Spontaneous splenic rupture in an acute leukemia patient with splenic tuberculosis: A case report". Molecular and Clinical Oncology 6.2 (2017): 209-213.
Chicago
Zhang, Y., Zhang, J., Chen, T., Zeng, H., Zhao, B., Zhang, Y., Zhou, X., Han, W., Hu, Y., Liu, F., Shan, Z., Gao, W., Zhou, H."Spontaneous splenic rupture in an acute leukemia patient with splenic tuberculosis: A case report". Molecular and Clinical Oncology 6, no. 2 (2017): 209-213. https://doi.org/10.3892/mco.2016.1120