Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model

  • Authors:
    • Jeeyoung Kim
    • Huijeong Ahn
    • Heung-Myong Woo
    • Eunsong Lee
    • Geun-Shik Lee
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  • Published online on: May 7, 2014     https://doi.org/10.3892/mmr.2014.2217
  • Pages: 329-335
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Abstract

Liver cancer is one of the most serious life-threatening diseases in the world. Although the rodent model of hepatocellar carcinoma (HCC) is commonly used, it is limited when considering preclinical applications, including transarterial chemoembolization. The pig is a more appropriate model for applying preclinical procedures as it has similar anatomical and physiological characteristics to humans. In the current study, transgenic fibroblasts were generated that overexpressed two proto-oncogenes specifically in hepatocytes. Porcine TGF-α and c-myc genes were isolated and these were linked with the porcine albumin promoter, which has exhibited selective activity in liver cells. Targeting vectors were introduced into the porcine fibroblasts using a liposome-mediated delivery system and the transgenic cell line was screened with 3 weeks of G-418 treatment. Selected vector‑positive colonies were further confirmed with polymerase chain reaction-based genotyping. Thus, the transgenic cell lines created in the current study should induce liver cancer in pig models following somatic cell nuclear transfer.
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July-2014
Volume 10 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kim J, Ahn H, Woo H, Lee E and Lee G: Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model. Mol Med Rep 10: 329-335, 2014.
APA
Kim, J., Ahn, H., Woo, H., Lee, E., & Lee, G. (2014). Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model. Molecular Medicine Reports, 10, 329-335. https://doi.org/10.3892/mmr.2014.2217
MLA
Kim, J., Ahn, H., Woo, H., Lee, E., Lee, G."Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model". Molecular Medicine Reports 10.1 (2014): 329-335.
Chicago
Kim, J., Ahn, H., Woo, H., Lee, E., Lee, G."Generation of liver-specific TGF-α and c-Myc-overexpressing fibroblasts for future creation of a liver cancer porcine model". Molecular Medicine Reports 10, no. 1 (2014): 329-335. https://doi.org/10.3892/mmr.2014.2217