Open Access

Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth

  • Authors:
    • Nan Zhang
    • Baoxing Chen
    • Wei Wang
    • Chao Chen
    • Jie Kang
    • Samuel Qinnan Deng
    • Bin Zhang
    • Shuwei Liu
    • Fabin Han
  • View Affiliations

  • Published online on: May 5, 2016     https://doi.org/10.3892/mmr.2016.5214
  • Pages: 95-102
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to isolate stem cells from human exfoliated deciduous teeth (SHEDs) and identify their phenotypes and multi‑lineage differentiation potential. Three SHED cell strains were successfully isolated from three exfoliated deciduous teeth from different human subjects using the outgrowth method. Flow cytometric analysis indicated that SHEDs displayed high expression of the mesenchymal cell markers CD73 and CD90 but low expression of the hematopoietic stem cell marker CD34. PCR analysis illustrated that SHEDs expressed the mesenchymal stem cell markers CD44, CD73 and CD90, the osteoblast markers Alpl, Runx2, CBFA1 and collagen Ⅰ, the cartilage cell markers Col10a1 and Acan, the adipose cell markers PPARγ2 and LPL, and the neuronal stem cell marker Nestin. In vitro induction experiments demonstrated the potential of the SHEDs for osteogenic, adipogenic and neurogenic differentiation. These SHED cells may be useful for further stem cell research and future therapeutic applications.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang N, Chen B, Wang W, Chen C, Kang J, Deng SQ, Zhang B, Liu S and Han F: Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth. Mol Med Rep 14: 95-102, 2016
APA
Zhang, N., Chen, B., Wang, W., Chen, C., Kang, J., Deng, S.Q. ... Han, F. (2016). Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth. Molecular Medicine Reports, 14, 95-102. https://doi.org/10.3892/mmr.2016.5214
MLA
Zhang, N., Chen, B., Wang, W., Chen, C., Kang, J., Deng, S. Q., Zhang, B., Liu, S., Han, F."Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth". Molecular Medicine Reports 14.1 (2016): 95-102.
Chicago
Zhang, N., Chen, B., Wang, W., Chen, C., Kang, J., Deng, S. Q., Zhang, B., Liu, S., Han, F."Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth". Molecular Medicine Reports 14, no. 1 (2016): 95-102. https://doi.org/10.3892/mmr.2016.5214