Effects of ischemic preconditioning on PDGF-BB expression in the gerbil hippocampal CA1 region following transient cerebral ischemia

Lee,Jae‑Chul; Kim,Yang  Hee; Lee,Tae‑Kyeong; Kim,In  Hye; Cho,Jeong  Hwi; Cho,Geum‑Sil; Shin,Bich‑Na; Park,Joon  Ha; Ahn,Ji  Hyeon; Shin,Myoung  Cheol; Cho,Jun  Hwi; Kang,Il  Jun; Won,Moo‑Ho; Seo,Jeong  Yeol

doi:10.3892/mmr.2017.6799

Effects of ischemic preconditioning on PDGF-BB expression in the gerbil hippocampal CA1 region following transient cerebral ischemia

Authors:
- Jae‑Chul Lee
- Yang Hee Kim
- Tae‑Kyeong Lee
- In Hye Kim
- Jeong Hwi Cho
- Geum‑Sil Cho
- Bich‑Na Shin
- Joon Ha Park
- Ji Hyeon Ahn
- Myoung Cheol Shin
- Jun Hwi Cho
- Il Jun Kang
- Moo‑Ho Won
- Jeong Yeol Seo
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Affiliations: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Pharmacology and Toxicology Department, Shinpoong Pharmaceutical Co., Ltd., Ansan, Gyeonggi 15610, Republic of Korea, Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea
Published online on: June 19, 2017 https://doi.org/10.3892/mmr.2017.6799
Pages: 1627-1634
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemic preconditioning (IPC) is induced by exposure to brief durations of transient ischemia, which results in ischemic tolerance to a subsequent longer or lethal period of ischemia. In the present study, the effects of IPC (2 min of transient cerebral ischemia) were examined on immunoreactivity of platelet‑derived growth factor (PDGF)‑BB and on neuroprotection in the gerbil hippocampal CA1 region following lethal transient cerebral ischemia (LTCI; 5 min of transient cerebral ischemia). IPC was subjected to a 2‑min sublethal ischemia and a LTCI was given 5‑min transient ischemia. The animals in all of the groups were given recovery times of 1, 2 and 5 days and change in PDGF‑BB immunoreactivity was examined as was the neuronal damage/death in the hippocampus induced by LTCI. LTCI induced a significant loss of pyramidal neurons in the hippocampal CA1 region 5 days after LTCI, and significantly decreased PDGF‑BB immunoreactivity in the CA1 pyramidal neurons from day 1 after LTCI. Conversely, IPC effectively protected the CA1 pyramidal neurons from LTCI and increased PDGF‑BB immunoreactivity in the CA1 pyramidal neurons post‑LTCI. In conclusion, the results demonstrated that LTCI significantly altered PDGF‑BB immunoreactivity in pyramidal neurons in the hippocampal CA1 region, whereas IPC increased the immunoreactivity. These findings indicated that PDGF‑BB may be associated with IPC‑mediated neuroprotection.

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