Open Access

Investigating the mechanisms of papillary thyroid carcinoma using transcriptome analysis

  • Authors:
    • Jie Qiu
    • Wenwei Zhang
    • Qingsheng Xia
    • Fuxue Liu
    • Shuwei Zhao
    • Kailing Zhang
    • Min Chen
    • Chuanshan Zang
    • Ruifeng Ge
    • Dapeng Liang
    • Yan Sun
  • View Affiliations

  • Published online on: August 24, 2017     https://doi.org/10.3892/mmr.2017.7346
  • Pages: 5954-5964
  • Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As the predominant thyroid cancer, papillary thyroid cancer (PTC) accounts for 75‑85% of thyroid cancer cases. This research aimed to investigate transcriptomic changes and key genes in PTC. Using RNA‑sequencing technology, the transcriptional profiles of 5 thyroid tumor tissues and 5 adjacent normal tissues were obtained. The single nucleotide polymorphisms (SNPs) were identified by SAMtools software and then annotated by ANNOVAR software. After differentially expressed genes (DEGs) were selected by edgR software, they were further investigated by enrichment analysis, protein domain analysis, and protein‑protein interaction (PPI) network analysis. Additionally, the potential gene fusion events were predicted using FusionMap software. A total of 70,172 SNPs and 2,686 DEGs in the tumor tissues, as well as 83,869 SNPs in the normal tissues were identified. In the PPI network, fibronectin 1 (FN1; degree=31) and transforming growth factor β receptor 1 (TGFβR1; degree=22) had higher degrees. A total of 7 PPI pairs containing the non‑synonymous risk SNP loci in the interaction domains were identified. Particularly, the interaction domains involved in the interactions of FN1 and 5 other proteins (such as FN1‑tenascin C, TNC) had non‑synonymous risk SNP loci. Furthermore, 11 and 4 gene fusion events were identified in all of the tumor tissues and normal tissues, respectively. Additionally, the NK2 homeobox 1‑surfactant associated 3 (NKX2‑1‑SFTA3) gene fusion was identified in both tumor and normal tissues. These results indicated that TGFβR1 and the NKX2‑1‑SFTA3 gene fusion may be involved in PTC. Furthermore, FN1 and TNC containing the non‑synonymous risk SNP loci might serve a role in PTC by interacting with each other.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Qiu J, Zhang W, Xia Q, Liu F, Zhao S, Zhang K, Chen M, Zang C, Ge R, Liang D, Liang D, et al: Investigating the mechanisms of papillary thyroid carcinoma using transcriptome analysis. Mol Med Rep 16: 5954-5964, 2017
APA
Qiu, J., Zhang, W., Xia, Q., Liu, F., Zhao, S., Zhang, K. ... Sun, Y. (2017). Investigating the mechanisms of papillary thyroid carcinoma using transcriptome analysis. Molecular Medicine Reports, 16, 5954-5964. https://doi.org/10.3892/mmr.2017.7346
MLA
Qiu, J., Zhang, W., Xia, Q., Liu, F., Zhao, S., Zhang, K., Chen, M., Zang, C., Ge, R., Liang, D., Sun, Y."Investigating the mechanisms of papillary thyroid carcinoma using transcriptome analysis". Molecular Medicine Reports 16.5 (2017): 5954-5964.
Chicago
Qiu, J., Zhang, W., Xia, Q., Liu, F., Zhao, S., Zhang, K., Chen, M., Zang, C., Ge, R., Liang, D., Sun, Y."Investigating the mechanisms of papillary thyroid carcinoma using transcriptome analysis". Molecular Medicine Reports 16, no. 5 (2017): 5954-5964. https://doi.org/10.3892/mmr.2017.7346