Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy

  • Authors:
    • Yong‑Li Yang
    • Shou‑Jun Guo
    • Zheng‑Xia Zhang
    • Yong Zhang
    • Ang Liu
    • Li‑Ling Xie
    • Yu‑Zhong Zheng
  • View Affiliations

  • Published online on: December 14, 2017     https://doi.org/10.3892/mmr.2017.8280
  • Pages: 3870-3876
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Abstract

A non-ionic water-soluble galactomannan, isolated from the seed endosperm of Crotalaria mucronata Desv., may be an ideal thickener to increase food viscosity. The present study hypothesized that the viscous property of Crotalaria galactomannan may be associated with its spatial structure. Therefore, the structure of Crotalaria galactomannan was elucidated using an atomic force microscope. The results of the present study demonstrated that the polysaccharide consisted of a D‑mannose backbone with D‑galactose branches, and the D‑mannose/D‑galactose ratio was 2.375:1. In the three‑dimensional structure of Crotalaria galactomannan, the helix was a common secondary structure, containing numerous ring structures of different sizes. In addition, multiple helixes may link together via hydrogen bonding and van der Waals forces, forming aggregations with small rings or spiral windings. The results of the present study indicated that the multiple‑branching construction of Crotalaria galactomannan may underlie its viscosity‑enhancing properties in the water phase.
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March-2018
Volume 17 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yang YL, Guo SJ, Zhang ZX, Zhang Y, Liu A, Xie LL and Zheng YZ: Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy. Mol Med Rep 17: 3870-3876, 2018
APA
Yang, Y., Guo, S., Zhang, Z., Zhang, Y., Liu, A., Xie, L., & Zheng, Y. (2018). Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy. Molecular Medicine Reports, 17, 3870-3876. https://doi.org/10.3892/mmr.2017.8280
MLA
Yang, Y., Guo, S., Zhang, Z., Zhang, Y., Liu, A., Xie, L., Zheng, Y."Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy". Molecular Medicine Reports 17.3 (2018): 3870-3876.
Chicago
Yang, Y., Guo, S., Zhang, Z., Zhang, Y., Liu, A., Xie, L., Zheng, Y."Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy". Molecular Medicine Reports 17, no. 3 (2018): 3870-3876. https://doi.org/10.3892/mmr.2017.8280