Hypoxia-induced increases in A549/CDDP cell drug resistance are reversed by RNA interference of HIF-1α expression

  • Authors:
    • Lingfeng Min
    • Qiong Chen
    • Shuya He
    • Shenggang Liu
    • Yun Ma
  • View Affiliations

  • Published online on: September 29, 2011     https://doi.org/10.3892/mmr.2011.604
  • Pages: 228-232
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Abstract

This study aimed to investigate the effects of knocking down hypoxia-inducible factor-1α (HIF-1α) through RNA interference on hypoxia-induced increases in drug resistance in A549/CDDP cells, and to study the underlying mechanisms. A small interfering RNA (siRNA) eukaryotic expression vector targeting HIF-1α was constructed and transfected into A549/CDDP cells treated with hypoxia. The mRNA and protein levels of HIF-1α, multidrug resistance-1 (MDR1), and multidrug resistance-associated protein (MRP) were determined by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry. Cell viability following treatment with cisplatin was determined by MTT assay. Hypoxia increased the resistance of A549/CDDP cells to cisplatin and this effect was reversed by the siRNA inhibition of HIF-1α expression. Expression of HIF-1α siRNA also downregulated HIF-1α, MDR1 and MRP mRNA, and protein expression in A549/CDDP cells treated with hypoxia (p<0.05). Hypoxia-induced resistance of A549/CDDP cells to cisplatin is reversed by the siRNA inhibition of HIF-1α expression. This effect may be mediated by a decreased expression of MDR1 and MRP.

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January 2012
Volume 5 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Min L, Chen Q, He S, Liu S and Ma Y: Hypoxia-induced increases in A549/CDDP cell drug resistance are reversed by RNA interference of HIF-1α expression. Mol Med Rep 5: 228-232, 2012.
APA
Min, L., Chen, Q., He, S., Liu, S., & Ma, Y. (2012). Hypoxia-induced increases in A549/CDDP cell drug resistance are reversed by RNA interference of HIF-1α expression. Molecular Medicine Reports, 5, 228-232. https://doi.org/10.3892/mmr.2011.604
MLA
Min, L., Chen, Q., He, S., Liu, S., Ma, Y."Hypoxia-induced increases in A549/CDDP cell drug resistance are reversed by RNA interference of HIF-1α expression". Molecular Medicine Reports 5.1 (2012): 228-232.
Chicago
Min, L., Chen, Q., He, S., Liu, S., Ma, Y."Hypoxia-induced increases in A549/CDDP cell drug resistance are reversed by RNA interference of HIF-1α expression". Molecular Medicine Reports 5, no. 1 (2012): 228-232. https://doi.org/10.3892/mmr.2011.604