Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells

  • Authors:
    • Yi-Qun Che
    • Peng Liu
    • Yue Wang
    • Chang-Gong Zhang
    • Ya-Ling Han
    • Di Shen
    • Ying Zhang
    • Cui-Ling Zheng
    • Jun Qi
    • Qing-Tao Wang
  • View Affiliations

  • Published online on: November 3, 2011     https://doi.org/10.3892/mmr.2011.663
  • Pages: 383-387
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The purpose of this study was to investigate the roles of bcl-2 chromosomal translocation and Bcl-2 protein expression in follicular lymphoma (FL) minimal bone marrow (BM) infiltration. We identified the same bcl-2/IgH fusion gene in paraffin-embedded lymph node (LN) samples and BM samples using immunohistochemistry (IHC), immunocytochemistry (ICC), cytologic morphology and fluorescence in situ hybridization (FISH). The presence of the Bcl-2/IgH fusion gene in the BM samples and paraffin-embedded LN samples from 56 patients with follicular lymphomas was detected using FISH. The Bcl-2 protein levels in BM and paraffin-embedded tissues were quantified using ICC and IHC, respectively. Approximately 78.6% (44/56) of the paraffin‑embedded LN tissue sections that underwent FISH analysis had a bcl-2/IgH translocation. The primary lesion was also positive for the bcl-2/IgH fusion gene, as were the BM minimal infiltrates. The bcl-2/IgH rearrangement occurred in 88.6% (39/44) of the BM specimens. The bcl-2/IgH recombination rate in stage III/IV cancers was significantly different to that observed in stage I/II cancers (p=0.041). In 59% (23/39) of the cases with t(14;18), Bcl-2 was found to be present as assessed by ICC. Positive Bcl-2 ICC staining and the t(14;18) translocation (as detected using FISH) were positively correlated (p=0.028). We then applied the FISH method to slides that had previously been morphologically evaluated using Wright-Giemsa staining; any slides with at least one abnormal cell were subjected to FISH analysis following staining. The assessment of bcl-2/IgH translocation status may contribute to the better detection of minimal BM infiltration by FL cells. Utilizing FISH and cytologic morphology techniques allows for earlier and more accessible BM examination.

Related Articles

Journal Cover

February 2012
Volume 5 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Che Y, Liu P, Wang Y, Zhang C, Han Y, Shen D, Zhang Y, Zheng C, Qi J, Wang Q, Wang Q, et al: Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells. Mol Med Rep 5: 383-387, 2012.
APA
Che, Y., Liu, P., Wang, Y., Zhang, C., Han, Y., Shen, D. ... Wang, Q. (2012). Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells. Molecular Medicine Reports, 5, 383-387. https://doi.org/10.3892/mmr.2011.663
MLA
Che, Y., Liu, P., Wang, Y., Zhang, C., Han, Y., Shen, D., Zhang, Y., Zheng, C., Qi, J., Wang, Q."Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells". Molecular Medicine Reports 5.2 (2012): 383-387.
Chicago
Che, Y., Liu, P., Wang, Y., Zhang, C., Han, Y., Shen, D., Zhang, Y., Zheng, C., Qi, J., Wang, Q."Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells". Molecular Medicine Reports 5, no. 2 (2012): 383-387. https://doi.org/10.3892/mmr.2011.663