Open Access

Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells

  • Authors:
    • Yanyu Li
    • Ling Liu
    • Zeyuan Yin
    • Hui Xu
    • Shuang Li
    • Wei Tao
    • Hui Cheng
    • Lei Du
    • Xueyuan Zhou
    • Bei Zhang
  • View Affiliations

  • Published online on: December 16, 2016     https://doi.org/10.3892/ol.2016.5511
  • Pages:567-572
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

0

Abstract

The aim of the study was to determine whether interleukin-8 (IL-8) affects human SKOV3 ovarian cancer cell migration and invasion by targeting silencing of IL-8 expression. Silencing small‑interfering RNA (siRNA) targeting IL-8 gene was constructed to infect SKOV3 cells by lentiviral vector. The expression of IL-8 and p-nuclear factor (NF)-κB protein was detected by western blot analysis. The wound scratch and Transwell tests were used to assay the cell migration and invasiveness of SKOV3 cells infected with lentiviral vector targeting IL-8 gene siRNA. The levels of IL-8 protein expressed by SKOV3 cells infected by lentiviral vector targeting IL-8 gene siRNA decreased by 72.3%. IL-8 (50 ng/ml) increased the ability of SKOV3 cells to suppress cell migration (p<0.01). Cisplatin and silencing of IL-8 achieved the ability to inhibit SKOV3 cell invasion (p<0.01), and 100 ng/ml concentration of IL-8 enhanced the ability of SKOV3 invasion (p<0.01). Silencing of IL-8 to a certain extent reduced the expression of p-NF-κB proteins, but it was not statistically significant. In conclusion, silencing of IL-8 may inhibit the migration and invasion of SKOV3 cells, which may be independent of the p-NF-κB protein.

Related Articles

Journal Cover

February 2017
Volume 13 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2015 Impact Factor: 1.482
Ranked #97/211 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Li, Y., Liu, L., Yin, Z., Xu, H., Li, S., Tao, W. ... Zhang, B. (2017). Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells. Oncology Letters, 13, 567-572. https://doi.org/10.3892/ol.2016.5511
MLA
Li, Y., Liu, L., Yin, Z., Xu, H., Li, S., Tao, W., Cheng, H., Du, L., Zhou, X., Zhang, B."Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells". Oncology Letters 13.2 (2017): 567-572.
Chicago
Li, Y., Liu, L., Yin, Z., Xu, H., Li, S., Tao, W., Cheng, H., Du, L., Zhou, X., Zhang, B."Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells". Oncology Letters 13, no. 2 (2017): 567-572. https://doi.org/10.3892/ol.2016.5511