Open Access

Danshensu, a major water‑soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice

  • Authors:
    • Hong‑Ying Cao
    • Rui‑Lin Ding
    • Meng Li
    • Mao‑Nan Yang
    • Ling‑Lin Yang
    • Jing‑Bo Wu
    • Bo Yang
    • Jing Wang
    • Cui‑Lian Luo
    • Qing‑Lian Wen
  • View Affiliations

  • Published online on: Friday, December 16, 2016
  • Pages:605-612 DOI: 10.3892/ol.2016.5508
  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The molecule 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid (danshensu), a herbal preparation used in traditional Chinese medicine, has been found to possess potential antitumor and anti‑angiogenesis effects. The aim of the present study was to investigate the efficacy of the combination of radiation therapy (RT) with danshensu in the treatment of Lewis lung carcinoma (LLC) xenografts, whilst exploring and evaluating the mechanism involved. In total, 8‑week old female C57BL/6J mice were randomly assigned into 3 groups to receive: RT, RT + cisplatin and RT + danshensu, respectively, when LLC reached 100‑150 mm3. Each group was divided into 7 subgroups according to the different irradiation doses that were administered. Tumor growth curves were created and the sensitization enhancement ratios of the drugs were calculated. The experiment was then repeated, and the 4 groups of tumor‑bearing mice were treated with natural saline, danshensu, RT + danshensu and RT, respectively. The mice were sacrificed on day 7, and tumor tissue and blood were collected to determine microvessel density, the expression of proangiogenic factors, and the levels of blood thromboxane B2 and 6‑keto‑prostaglandin‑F1α. Tumor hypoxia was also detected using in vivo fluorescence imaging. With respect to LLC xenografts, treatment with danshensu + RT significantly enhanced the effects of tumor growth inhibition (P<0.05). Furthermore, tumor vasculature was remodeled and microcirculation was improved, which significantly reduced tumor hypoxia (P<0.05). The present study demonstrated that danshensu significantly enhanced the radioresponse of LLC xenografts in mice. The mechanism involved may be associated with the alleviation of tumor cell hypoxia following treatment with danshensu + RT, caused by the improvement of tumor microcirculation and the remodeling of tumor vasculature.

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February 2017
Volume 13 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2015 Impact Factor: 1.482
Ranked #97/211 Oncology
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APA
Cao, H., Ding, R., Li, M., Yang, M., Yang, L., Wu, J. ... Wen, Q. (2017). Danshensu, a major water‑soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice. Oncology Letters, 13, 605-612. http://dx.doi.org/10.3892/ol.2016.5508
MLA
Cao, H., Ding, R., Li, M., Yang, M., Yang, L., Wu, J., Yang, B., Wang, J., Luo, C., Wen, Q."Danshensu, a major water‑soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice". Oncology Letters 13.2 (2017): 605-612.
Chicago
Cao, H., Ding, R., Li, M., Yang, M., Yang, L., Wu, J., Yang, B., Wang, J., Luo, C., Wen, Q."Danshensu, a major water‑soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice". Oncology Letters 13, no. 2 (2017): 605-612. http://dx.doi.org/10.3892/ol.2016.5508