Anti-metastatic activity of fangchinoline in human gastric cancer AGS cells

Chen,Zhengrong; He,Tengfei; Zhao,Kui; Xing,Chungen

doi:10.3892/ol.2016.5457

Anti-metastatic activity of fangchinoline in human gastric cancer AGS cells

Authors:
- Zhengrong Chen
- Tengfei He
- Kui Zhao
- Chungen Xing
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Affiliations: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215007, P.R. China
Published online on: December 2, 2016 https://doi.org/10.3892/ol.2016.5457
Pages: 655-660
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fangchinoline (FCL) is an active component isolated from the traditional medicinal plant Stephania tetrandra S. Moore, and has been reported to possess anti‑cancer functions in several types of cancers; however, the effect of FCL on gastric cancer metastasis and its underlying molecular mechanisms remain unknown. The current study aimed to investigate the effect of FCL on the cell migration and invasion of human metastatic gastric cancer AGS cells and its mechanisms. Our study demonstrates that FCL dosage dependently suppressed the adhesion, migration and invasion capacities of human gastric cancer AGS cells without obvious cytotoxic effects. Reverse transcription‑polymerase chain reaction and western blot assays demonstrated that FCL greatly inhibited the expression of matrix metalloproteinase (MMP)‑2 and MMP‑9 at both the mRNA and protein levels, while it significantly increased the expression of tissue inhibitor of metalloproteinase (TIMP) 1 and TIMP2 messenger RNAs. Our results also indicated that FCL repressed the phosphorylation of AKT in gastric cancer AGS cells. In summary, FCL may exert its anti‑metastatic property in human gastric cancer cells in vitro by suppression of MMP‑2 and MMP‑9, increase of TIMP1 and TIMP2 genes, and inhibition of AKT phosphorylation. FCL may be a drug candidate for the treatment of gastric cancer metastasis.

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