Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells

Zhao,Guanjie; Wu,Minfei; Wang,Xiaofeng; Du,Zhenwu; Zhang,Guizhen

doi:10.3892/ol.2017.6748

Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells

Authors:
- Guanjie Zhao
- Minfei Wu
- Xiaofeng Wang
- Zhenwu Du
- Guizhen Zhang
View Affiliations

Affiliations: Research Center of The Second Hospital, Jilin University, Changchun, Jilin 130041, P.R. China, Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Published online on: August 11, 2017 https://doi.org/10.3892/ol.2017.6748
Pages: 4772-4778
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to examine the effect of fatty acid binding protein-5 (FABP-5) gene on the proliferation, apoptosis and invasion of human gastric cancer SGC-7901 cells. The viability, apoptosis and cell invasion of SGC-7901 cells before and after FABP5 knockdown were taken as the study objects, design and synthesis of siRNA interference sequence were conducted according to FABP-5 mRNA coding sequences, and SGC-7901 cells were transiently transfected. The human gastric cancer SGC-7901 cells were divided into three groups: FABP-5 siRNA group, negative control group and blank control group. FABP-5 gene mRNA and protein expression levels were detected by RT-PCR and western blot analysis. The CCK-8 assay was used to detect in vitro cell proliferation, flow cytometry (FCM) was used to detect changes in cell cycle and apoptosis in each group, TUNEL staining was used to detect apoptosis in each group, and the cell invasion chamber assay was used to detect cell invasiveness in each group. Each test was repeated three times. The results of the RT-PCR and western blot analysis showed that, expression of FABP-5 mRNA and protein in the FABP-5 siRNA group was significantly decreased compared with the negative and blank control groups. The cell growth rate in the FABP-5 siRNA group was significantly retarded, cell cycle was arrested in G0/G1 phase, the number of cells in S phase was reduced, and compared with the negative and blank control groups, the apoptotic rate in the FABP-5 siRNA group was significantly increased (P<0.01), while proliferation and invasiveness were significantly decreased (P<0.05). In conclusion, specific FABP-5 gene silencing may reduce the invasiveness of gastric cancer cells, inhibit cell proliferation, and arrest cell cycle in G0/G1 phase, resulting in a significant increase in apoptosis.

View Figures

View References

1	Zhu YL, Yang L, Sui ZQ, Liu L and Du JF: Clinicopathological features and prognosis of Borrmann type IV gastric cancer. J BUON. 21:1471–1475. 2016.PubMed/NCBI
2	Yildiz B, Etiz D, Dal P, Junushova B, Pasaoglu O, Yilmaz E, Erkasap S and Dincer M: Tumor deposits: Prognostic significance in gastric cancer patients. J BUON. 21:1476–1481. 2016.PubMed/NCBI
3	Veerkamp JH, Maatman RG and Prinsen CF: Fatty acid-binding proteins: Structural and functional diversity. Biochem Soc Trans. 20:801–805. 1992. View Article : Google Scholar : PubMed/NCBI
4	Münz M, Zeidler R and Gires O: The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP. Cancer Lett. 225:151–157. 2005. View Article : Google Scholar : PubMed/NCBI
5	Uma RS, Naresh KN, D'Cruz AK, Mulherkar R and Borges AM: Metastasis of squamous cell carcinoma of the oral tongue is associated with down-regulation of epidermal fatty acid binding protein (E-FABP). Oral Oncol. 43:27–32. 2007. View Article : Google Scholar : PubMed/NCBI
6	Liu RZ, Graham K, Glubrecht DD, Germain DR, Mackey JR and Godbout R: Association of FABP5 expression with poor survival in triple-negative breast cancer: implication for retinoic acid therapy. Am J Pathol. 178:997–1008. 2011. View Article : Google Scholar : PubMed/NCBI
7	Smith S, Witkowski A and Joshi AK: Structural and functional organization of the animal fatty acid synthase. Prog Lipid Res. 42:289–317. 2003. View Article : Google Scholar : PubMed/NCBI
8	Chmurzyńska A: The multigene family of fatty acid-binding proteins (FABPs): Function, structure and polymorphism. J Appl Genet. 47:39–48. 2006. View Article : Google Scholar : PubMed/NCBI
9	Thumser AE, Moore JB and Plant NJ: Fatty acid binding proteins: Tissue-specific functions in health and disease. Curr Opin Clin Nutr Metab Care. 17:124–129. 2014. View Article : Google Scholar : PubMed/NCBI
10	Zimmerman AW, van Moerkerk HT and Veerkamp JH: Ligand specificity and conformational stability of human fatty acid-binding proteins. Int J Biochem Cell Biol. 33:865–876. 2001. View Article : Google Scholar : PubMed/NCBI
11	Kawamura T, Kanno R, Fujii H and Suzuki T: Expression of liver-type fatty-acid-binding protein, fatty acid synthase and vascular endothelial growth factor in human lung carcinoma. Pathobiology. 72:233–240. 2005. View Article : Google Scholar : PubMed/NCBI
12	Lawrie LC, Dundas SR, Curran S and Murray GI: Liver fatty acid binding protein expression in colorectal neoplasia. Br J Cancer. 90:1955–1960. 2004. View Article : Google Scholar : PubMed/NCBI
13	Hammamieh R, Chakraborty N, Barmada M, Das R and Jett M: Expression patterns of fatty acid binding proteins in breast cancer cells. J Exp Ther Oncol. 5:133–143. 2005.PubMed/NCBI
14	Tölle A, Suhail S, Jung M, Jung K and Stephan C: Fatty acid binding proteins (FABPs) in prostate, bladder and kidney cancer cell lines and the use of IL-FABP as survival predictor in patients with renal cell carcinoma. BMC Cancer. 11:3022011. View Article : Google Scholar : PubMed/NCBI
15	Hashimoto T, Kusakabe T, Watanabe K, Sugino T, Fukuda T, Nashimoto A, Honma K, Sato Y, Kimura H, Fujii H, et al: Liver-type fatty acid-binding protein is highly expressed in intestinal metaplasia and in a subset of carcinomas of the stomach without association with the fatty acid synthase status in the carcinoma. Pathobiology. 71:115–122. 2004. View Article : Google Scholar : PubMed/NCBI
16	Armstrong EH, Goswami D, Griffin PR, Noy N and Ortlund EA: Structural basis for ligand regulation of the fatty acid-binding protein 5, peroxisome proliferator-activated receptor β/δ (FABP5-PPARβ/δ) signaling pathway. J Biol Chem. 289:14941–14954. 2014. View Article : Google Scholar : PubMed/NCBI
17	Levi L, Lobo G, Doud MK, von Lintig J, Seachrist D, Tochtrop GP and Noy N: Genetic ablation of the fatty acid-binding protein FABP5 suppresses HER2-induced mammary tumorigenesis. Cancer Res. 73:4770–4780. 2013. View Article : Google Scholar : PubMed/NCBI
18	Harris FT, Rahman SM, Hassanein M, Qian J, Hoeksema MD, Chen H, Eisenberg R, Chaurand P, Caprioli RM, Shiota M, et al: Acyl-coenzyme A-binding protein regulates Beta-oxidation required for growth and survival of non-small cell lung cancer. Cancer Prev Res (Phila). 7:748–757. 2014. View Article : Google Scholar : PubMed/NCBI
19	Liu Q, Wang S, Xu H and Zhang S: Expressions and significances of CRABPII and E-FABP in non-small cell lung cancer. Zhongguo Fei Ai Za Zhi. 16:12–19. 2013.(In Chinese). PubMed/NCBI
20	Celis JE, Rasmussen HH, Vorum H, Madsen P, Honoré B, Wolf H and Orntoft TF: Bladder squamous cell carcinomas express psoriasin and externalize it to the urine. J Urol. 155:2105–2112. 1996. View Article : Google Scholar : PubMed/NCBI
21	Adamson J, Morgan EA, Beesley C, Mei Y, Foster CS, Fujii H, Rudland PS, Smith PH and Ke Y: High-level expression of cutaneous fatty acid-binding protein in prostatic carcinomas and its effect on tumorigenicity. Oncogene. 22:2739–2749. 2003. View Article : Google Scholar : PubMed/NCBI
22	Morgan EA, Forootan SS, Adamson J, Foster CS, Fujii H, Igarashi M, Beesley C, Smith PH and Ke Y: Expression of cutaneous fatty acid-binding protein (C-FABP) in prostate cancer: Potential prognostic marker and target for tumourigenicity-suppression. Int J Oncol. 32:767–775. 2008.PubMed/NCBI
23	Jeong CY, Hah YS, Cho BI, Lee SM, Joo YT, Jung EJ, Jeong SH, Lee YJ, Choi SK, Ha WS, et al: Fatty acid-binding protein 5 promotes cell proliferation and invasion in human intrahepatic cholangiocarcinoma. Oncol Rep. 28:1283–1292. 2012. View Article : Google Scholar : PubMed/NCBI
24	Fang LY, Wong TY, Chiang WF and Chen YL: Fatty-acid-binding protein 5 promotes cell proliferation and invasion in oral squamous cell carcinoma. J Oral Pathol Med. 39:342–348. 2010. View Article : Google Scholar : PubMed/NCBI
25	Rauch J, Ahlemann M, Schaffrik M, Mack B, Ertongur S, Andratschke M, Zeidler R, Lang S and Gires O: Allogenic antibody-mediated identification of head and neck cancer antigens. Biochem Biophys Res Commun. 323:156–162. 2004. View Article : Google Scholar : PubMed/NCBI
26	Li Z, Huang C, Bai S, Pan X, Zhou R, Wei Y and Zhao X: Prognostic evaluation of epidermal fatty acid-binding protein and calcyphosine, two proteins implicated in endometrial cancer using a proteomic approach. Int J Cancer. 123:2377–2383. 2008. View Article : Google Scholar : PubMed/NCBI
27	Brouard MC, Saurat JH, Ghanem G and Siegenthaler G: Urinary excretion of epidermal-type fatty acid-binding protein and S100A7 protein in patients with cutaneous melanoma. Melanoma Res. 12:627–631. 2002. View Article : Google Scholar : PubMed/NCBI
28	Ogawa R, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Mori Y, Mori R, Tomoda K, Katada T, Harada K, et al: Identification of candidate genes involved in the radiosensitivity of esophageal cancer cells by microarray analysis. Dis Esophagus. 21:288–297. 2008. View Article : Google Scholar : PubMed/NCBI