Downregulated cytoplasmic polyadenylation element-binding protein-4 is associated with the carcinogenesis of head and neck squamous cell carcinoma

Zeng,Manli; Li,Fen; Wang,Lei; Chen,Chen; Huang,Xiaolin; Wu,Xingyu; She,Wensheng; Zhou,Lin; Tao,Zezhang

doi:10.3892/ol.2017.7661

Downregulated cytoplasmic polyadenylation element-binding protein-4 is associated with the carcinogenesis of head and neck squamous cell carcinoma

Authors:
- Manli Zeng
- Fen Li
- Lei Wang
- Chen Chen
- Xiaolin Huang
- Xingyu Wu
- Wensheng She
- Lin Zhou
- Zezhang Tao
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Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Ezhou Central Hospital, Ezhou, Hubei 436000, P.R. China
Published online on: December 20, 2017 https://doi.org/10.3892/ol.2017.7661
Pages: 3226-3232

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Abstract

Cytoplasmic polyadenylation element‑binding protein‑4 (CPEB4) is involved in several biological processes that are associated with cancer progression. However, it remains unknown whether CPEB4 expression levels are associated with head and neck squamous cell carcinoma (HNSCC). The aim of the present study was to explore the potential function of CPEB4 in HNSCC. The expression of CPEB4 was analyzed in HNSCC from six Gene Expression Omnibus (GEO) datasets. Immunohistochemical staining was conducted to examine CPEB4 protein levels in an HNSCC tissue microarray (TMA). According to the GEO dataset analyses, CPEB4 gene expression was downregulated in HNSCC compared with normal samples (P<0.05). Notably, a statistical difference was observed between different tumor grades (P<0.05). Furthermore, the methylation of the CPEB4 gene in HNSCC was significantly increased compared with that observed in normal samples (P<0.01). The outcome from the TMA demonstrated that CPEB4 protein expression in human HNSCC tumors was significantly decreased compared with normal samples (P<0.05). In addition, the expression of CPEB4 protein was negatively associated with histological grades of HNSCC (P<0.05). The results from the present study suggested that CPEB4 may function as a tumor suppressor gene in HNSCC, which identifies the potential value of CPEB4 in predicting prognosis of HNSCC. Hypermethylation of the CPEB4 gene may be responsible for the downregulation of CPEB4 expression in HNSCC and result in tumorigenesis.

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