Gene expression analyses associated with malignant phenotypes of metastatic sub-clones derived from a mouse oral squamous cell carcinoma Sq-1979 cell line

Adachi,Mitsutaka; Mizuno‑Kamiya,Masako; Takayama,Eiji; Kawaki,Harumi; Inagaki,Toshihiro; Sumi,Shigeki; Motohashi,Masayuki; Muramatsu,Yasunori; Sumitomo,Shin‑Ichiro; Shikimori,Michio; Yamazaki,Yutaka; Kondoh,Nobuo

doi:10.3892/ol.2017.7648

Gene expression analyses associated with malignant phenotypes of metastatic sub-clones derived from a mouse oral squamous cell carcinoma Sq-1979 cell line

Authors:
- Mitsutaka Adachi
- Masako Mizuno‑Kamiya
- Eiji Takayama
- Harumi Kawaki
- Toshihiro Inagaki
- Shigeki Sumi
- Masayuki Motohashi
- Yasunori Muramatsu
- Shin‑Ichiro Sumitomo
- Michio Shikimori
- Yutaka Yamazaki
- Nobuo Kondoh
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Affiliations: Department of Oral Biochemistry, Asahi University School of Dentistry, Mizuho, Gifu 501‑0296, Japan, Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Mizuho, Gifu 501‑0296, Japan, Dentistry and Oral Maxillofacial Surgery Unit, Hokuriku Central Hospital of Japan Mutual Aid Association of Public School Teachers, Oyabe, Toyama 932‑8503, Japan, Department of Gerontology, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060‑8586, Japan
Published online on: December 19, 2017 https://doi.org/10.3892/ol.2017.7648
Pages: 3350-3356

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Abstract

To elucidate the genetic events that occur during the development of OSCC, the present study established a model of oral malignancy using a mouse oral squamous cell carcinoma (OSCC) Sq‑1979 cell line. Sq‑1979 cells were implanted into syngeneic C3H mice. Subsequently, 233 cells and metastatic sub‑clones (L cells) from primary OSCC, as well as the metastasized lymph node tissues of Sq‑1979‑implanted mice were established. Compared with parental Sq‑1979 and 233 cells, the majority of L cells exhibited a higher proliferation rate and transplantability, and conferred a lower survival rate on the implanted mice. To investigate the genetic background of L cells, preferentially expressed genes in L cells were identified by cDNA microarray and reverse transcription‑polymerase chain reaction analyses. The expression of FYN‑binding protein (Fyb), solute carrier family 16 member 13 (Slc16a13), keratin 7, transmembrane portion 173 and Slc44a3 mRNAs was significantly elevated in L cells compared with that in Sq1979 and 233 cells. The mRNA expression was also evaluated in human OSCC and leukoplakia (LP) tissues. Among the 5 aforementioned mRNAs, the expression of FYB and SLC16A13 was significantly higher in OSCC than in LP tissues. Furthermore, the expression of SLC16A13 mRNA was significantly elevated in highly invasive OSCCs, which were classified as grades 3 and 4 by the Yamamoto‑Kohama (YK) classification of invasion, compared with those in lower grades (YK‑1 and ‑2). The model proposed in the present study could thus describe essential marker genes for the diagnosis of oral malignancies.

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