Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues

  • Authors:
    • Axel S. Merseburger
    • Markus A. Kuczyk
    • Jurgen Serth
    • Carsten Bokemeyer
    • Denise Y. Young
    • Leon Sun
    • Roger R. Connelly
    • David G. McLeod
    • Fathollah K. Mostofi
    • Shiv K. Srivastava
    • A. Stenzl
    • Judd W. Moul
    • Isabell A. Sesterhenn
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/or.10.1.223
  • Pages: 223-228
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Abstract

Several investigators have reported the correlation of p53 and bcl-2 immunoreactivity with post operative prostate specific antigen (PSA) recurrence. Focal and or clustered expression is typical for these biomarkers. The purpose of this study was to compare the effectiveness of tissue microarrays to detect p53 and bcl-2 overexpression and their prognostic significance. Tissue microarrays (TMA) of 99 patients with mean follow-up of 61 months contained 760 samples from 241 carcinomas, 431 benign glands, and 88 foci of prostatic intraepithelial neoplasia (PIN). Overexpression of p53 was seen in 43.3% of 97 patients, whereas bcl-2 overexpression was noted in 23.7% of 97 patients using TMA technology, compared to 66.0% and 26.9%, respectively in the corresponding radical prostatectomy samples. The tissue microarray technology is a powerful tool to study the multifocal and heterogeneous nature of prostate cancer. However, the prognostic value of p53 and bcl-2 could not be confirmed using this technology in contrast to radical prostatectomy sections. The TMA technique is probably more informative and reliable in evaluating the prognostic value of homogeneously expressed biomarkers.

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January-February 2003
Volume 10 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Merseburger AS, Kuczyk MA, Serth J, Bokemeyer C, Young DY, Sun L, Connelly RR, McLeod DG, Mostofi FK, Srivastava SK, Srivastava SK, et al: Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues. Oncol Rep 10: 223-228, 2003.
APA
Merseburger, A.S., Kuczyk, M.A., Serth, J., Bokemeyer, C., Young, D.Y., Sun, L. ... Sesterhenn, I.A. (2003). Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues. Oncology Reports, 10, 223-228. https://doi.org/10.3892/or.10.1.223
MLA
Merseburger, A. S., Kuczyk, M. A., Serth, J., Bokemeyer, C., Young, D. Y., Sun, L., Connelly, R. R., McLeod, D. G., Mostofi, F. K., Srivastava, S. K., Stenzl, A., Moul, J. W., Sesterhenn, I. A."Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues". Oncology Reports 10.1 (2003): 223-228.
Chicago
Merseburger, A. S., Kuczyk, M. A., Serth, J., Bokemeyer, C., Young, D. Y., Sun, L., Connelly, R. R., McLeod, D. G., Mostofi, F. K., Srivastava, S. K., Stenzl, A., Moul, J. W., Sesterhenn, I. A."Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues". Oncology Reports 10, no. 1 (2003): 223-228. https://doi.org/10.3892/or.10.1.223