Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas

  • Authors:
    • Lynne W. Elmore
    • Robert Forsythe
    • Heidi Forsythe
    • A. Taylor Bright
    • Suhail Nasim
    • Kanendori Endo
    • Shawn E. Holt
  • View Affiliations

  • Published online on: September 1, 2008     https://doi.org/10.3892/or_00000049
  • Pages: 613-617
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Abstract

Over 90% of prostate cancers express telomerase activity. In an experimental model, hsp90 and p23, which are necessary for telomerase assembly and function, dramatically increase during tumorigenic conversion. We immunohistochemically analyzed 60 prostate carcinomas, 50 prostatic intraepithelial neoplasias (PIN) and 25 benign prostatic tissues to determine whether hsp90/p23 expression correlates with advancing stage and whether chaperone distribution overlaps with hTERT, the catalytic component of telomerase. Strong expression of hsp90/p23 was detected in ≈95% of PIN and carcinomas without relationship to Gleason score. While hsp90/p23 immunostaining was predominantly diffuse and cytoplasmic, nuclear immunoreactivity was observed in several moderate-to-high grade carcinomas, and those carcinomas with nuclear chaperone staining exhibited detectable hTERT. Our data suggest enhanced chaperone-mediated telomerase assembly as a mechanism for increased activity in advanced prostate carcinomas, stable association between chaperones and telomerase in vivo, and utility for chaperone immunostaining to identify focal PIN in the context of widespread hyperplasia.

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September 2008
Volume 20 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Elmore LW, Forsythe R, Forsythe H, Taylor Bright A, Nasim S, Endo K and Holt SE: Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas. Oncol Rep 20: 613-617, 2008.
APA
Elmore, L.W., Forsythe, R., Forsythe, H., Taylor Bright, A., Nasim, S., Endo, K., & Holt, S.E. (2008). Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas. Oncology Reports, 20, 613-617. https://doi.org/10.3892/or_00000049
MLA
Elmore, L. W., Forsythe, R., Forsythe, H., Taylor Bright, A., Nasim, S., Endo, K., Holt, S. E."Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas". Oncology Reports 20.3 (2008): 613-617.
Chicago
Elmore, L. W., Forsythe, R., Forsythe, H., Taylor Bright, A., Nasim, S., Endo, K., Holt, S. E."Overexpression of telomerase-associated chaperone proteins in prostatic intraepithelial neoplasia and carcinomas". Oncology Reports 20, no. 3 (2008): 613-617. https://doi.org/10.3892/or_00000049