Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer

  • Authors:
    • Xiao-Bo Long
    • Guang-Bin Sun
    • Shuang Hu
    • Geng-Tian Liang
    • Nan Wang
    • Xin-Hao Zhang
    • Ping-Ping Cao
    • Hong-Tao Zhen
    • Yong-Hua Cui
    • Zheng Liu
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/or_00000554
  • Pages: 1189-1195
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Abstract

MicroRNAs (miRNAs) are endogenously expressed non-coding RNAs, which are involved in the gene expression regulation. Lethal-7a (let-7a) is a founding member of miRNA family and recently it was found to be associated with several cancers, such as lung and colon cancers. In the present study, we found that let-7a miRNA expression was significantly downregulated both in human laryngeal squamous cancer tissues and in Hep-2 cells, a laryngeal cancer cell line, as compared with adjacent normal tissues and BEAS-2B cells, respectively. Moreover, we found that let-7a expression levels were significantly further decreased in non-differentiated (G3) cancer tissues as compared with moderately and well differentiated cancer tissues (G2 and G1), although no significant difference in let-7a expression levels between the cancer specimens with different T stages or specimens from patients with different lymph node metastasis status was revealed. In Hep-2 cells, let-7a mimics transfection markedly suppressed proliferation and induced apoptosis of Hep-2 cells under the treatment of diamminedichloroplatinum or not and downregulated RAS and c-MYC protein expression without affecting the mRNA levels. In parallel, RAS and c-MYC protein levels were found significantly upregulated only in cancer tissues with downregulated let-7a expression. Thus, we propose that let-7a may be a tumor suppressor in laryngeal cancer by inhibiting cell growth, inducing cell apoptosis and downregulating the oncogenes expression.

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November 2009
Volume 22 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Long X, Sun G, Hu S, Liang G, Wang N, Zhang X, Cao P, Zhen H, Cui Y, Liu Z, Liu Z, et al: Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer. Oncol Rep 22: 1189-1195, 2009.
APA
Long, X., Sun, G., Hu, S., Liang, G., Wang, N., Zhang, X. ... Liu, Z. (2009). Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer. Oncology Reports, 22, 1189-1195. https://doi.org/10.3892/or_00000554
MLA
Long, X., Sun, G., Hu, S., Liang, G., Wang, N., Zhang, X., Cao, P., Zhen, H., Cui, Y., Liu, Z."Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer". Oncology Reports 22.5 (2009): 1189-1195.
Chicago
Long, X., Sun, G., Hu, S., Liang, G., Wang, N., Zhang, X., Cao, P., Zhen, H., Cui, Y., Liu, Z."Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer". Oncology Reports 22, no. 5 (2009): 1189-1195. https://doi.org/10.3892/or_00000554