ARL6IP1 mediates cisplatin-induced apoptosis in CaSki cervical cancer cells

  • Authors:
    • Fengjie Guo
    • Yalin Li
    • Yan Liu
    • Jiajia Wang
    • Guancheng Li
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/or_00000783
  • Pages: 1449-1455
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Abstract

Cisplatin has been shown to induce apoptosis in various types of cancer cells. Despite the great efficacy at treating certain kinds of cancers, cisplatin introduced into clinical use shows side effects and the acquisition or presence of resistance to the drug. Thus, it is important that we further understand the anti-cancer mechanism of cisplatin with the goal of enhancing its efficacy. ADP-ribosylation factor-like 6 interacting protein 1 (ARL6IP1) is an apoptotic regulator. We studied cisplatin-induced apoptosis with suppression of ARL6IP1 expression in CaSki cervical cancer cells. Exogenous expression of ARL6IP1 suppressed cisplatin-induced apoptosis in CaSki cells, and siRNA-induced silencing of ARL6IP1 triggered apoptosis in CaSki cells even in the absence of other apoptotic stimuli. Cisplatin treatment induced caspase-3, -9, p53, Bax, NF-κB and MAPK expression, and suppressed Bcl-2 and Bcl-xl expression, whereas cells transfected with pcDNA3.1-ARL6IP1 showed lower levels of cisplatin-induced caspase-3, -9, p53, Bax, NF-κB and MAPK up-regulation and higher levels of cisplatin-suppressed Bcl-2 and Bcl-xl down-regulation. These novel findings collectively suggest that ARL6IP1 may play a key role in cisplatin-induced apoptosis in CaSki cervical cancer cells by regulating the expression of apoptosis-associated proteins such as caspase-3, -9, p53, NF-κB, MAPK, Bcl-2, Bcl-xl, and Bax.

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May 2010
Volume 23 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Guo F, Li Y, Liu Y, Wang J and Li G: ARL6IP1 mediates cisplatin-induced apoptosis in CaSki cervical cancer cells . Oncol Rep 23: 1449-1455, 2010.
APA
Guo, F., Li, Y., Liu, Y., Wang, J., & Li, G. (2010). ARL6IP1 mediates cisplatin-induced apoptosis in CaSki cervical cancer cells . Oncology Reports, 23, 1449-1455. https://doi.org/10.3892/or_00000783
MLA
Guo, F., Li, Y., Liu, Y., Wang, J., Li, G."ARL6IP1 mediates cisplatin-induced apoptosis in CaSki cervical cancer cells ". Oncology Reports 23.5 (2010): 1449-1455.
Chicago
Guo, F., Li, Y., Liu, Y., Wang, J., Li, G."ARL6IP1 mediates cisplatin-induced apoptosis in CaSki cervical cancer cells ". Oncology Reports 23, no. 5 (2010): 1449-1455. https://doi.org/10.3892/or_00000783